Abstract
This chapter describes protocols for mass spectrometry (MS) applied to the characterization of ganglioside structures and the determination of ganglioside contents. Matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) are often used to ionize biological materials and this chapter covers three protocols for atmospheric pressure MALDI MS (AP-MALDI MS), liquid chromatography-ESI MS (LC-ESI MS), and LC-ESI MS with multiple reaction monitoring (MRM). Purified gangliosides were used in AP-MALDI MS analyses while crude preparations of gangliosides were subjected to LC-ESI MS and LC-ESI MS with MRM. The LC protocol includes conditions for both reversed-phase and normal-phase column chromatography.
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References
Simons K, Ikonen E (1997) Functional rafts in cell membranes. Nature 387(6633):569–572. https://doi.org/10.1038/42408
Aureli M, Grassi S, Sonnino S, Prinetti A (2016) Isolation and analysis of detergent-resistant membrane fractions. Methods Mol Biol 1376:107–131. https://doi.org/10.1007/978-1-4939-3170-5_10
Park H, Zhou Y, Costello CE (2014) Direct analysis of sialylated or sulfated glycosphingolipids and other polar and neutral lipids using TLC-MS interfaces. J Lipid Res 55(4):773–781. https://doi.org/10.1194/jlr.D046128
Levery SB (2005) Glycosphingolipid structural analysis and glycosphingolipidomics. Methods Enzymol 405:300–369. https://doi.org/10.1016/S0076-6879(05)05012-3
Ito E, Tominaga A, Waki H, Miseki K, Tomioka A, Nakajima K, Kakehi K, Suzuki M, Taniguchi N, Suzuki A (2012) Structural characterization of monosialo-, disialo- and trisialo-gangliosides by negative ion AP-MALDI-QIT-TOF mass spectrometry with MS(n) switching. Neurochem Res 37(6):1315–1324. https://doi.org/10.1007/s11064-012-0735-z
Nagafuku M, Okuyama K, Onimaru Y, Suzuki A, Odagiri Y, Yamashita T, Iwasaki K, Fujiwara M, Takayanagi M, Ohno I, Inokuchi J (2012) CD4 and CD8 T cells require different membrane gangliosides for activation. Proc Natl Acad Sci U S A 109(6):E336–E342. https://doi.org/10.1073/pnas.1114965109
Oikawa N, Hatsuta H, Murayama S, Suzuki A, Yanagisawa K (2014) Influence of APOE genotype and the presence of Alzheimer’s pathology on synaptic membrane lipids of human brains. J Neurosci Res 92(5):641–650. https://doi.org/10.1002/jnr.23341
Oikawa N, Matsubara T, Fukuda R, Yasumori H, Hatsuta H, Murayama S, Sato T, Suzuki A, Yanagisawa K (2015) Imbalance in fatty-acid-chain length of gangliosides triggers Alzheimer amyloid deposition in the precuneus. PLoS One 10(3):e0121356. https://doi.org/10.1371/journal.pone.0121356
Veillon L, Go S, Matsuyama W, Suzuki A, Nagasaki M, Yatomi Y, Inokuchi J (2015) Identification of ganglioside GM3 molecular species in human serum associated with risk factors of metabolic syndrome. PLoS One 10(6):e0129645. https://doi.org/10.1371/journal.pone.0129645
Sturgill ER, Aoki K, Lopez PH, Colacurcio D, Vajn K, Lorenzini I, Majic S, Yang WH, Heffer M, Tiemeyer M, Marth JD, Schnaar RL (2012) Biosynthesis of the major brain gangliosides GD1a and GT1b. Glycobiology 22(10):1289–1301. https://doi.org/10.1093/glycob/cws103
Ikeda K, Taguchi R (2010) Highly sensitive localization analysis of gangliosides and sulfatides including structural isomers in mouse cerebellum sections by combination of laser microdissection and hydrophilic interaction liquid chromatography/electrospray ionization mass spectrometry with theoretically expanded multiple reaction monitoring. Rapid Commun Mass Spectr 24(20):2957–2965. https://doi.org/10.1002/rcm.4716
Ikeda K, Shimizu T, Taguchi R (2008) Targeted analysis of ganglioside and sulfatide molecular species by LC/ESI-MS/MS with theoretically expanded multiple reaction monitoring. J Lipid Res 49(12):2678–2689. https://doi.org/10.1194/jlr.D800038-JLR200
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Suzuki, A., Suzuki, M., Ito, E., Nitta, T., Inokuchi, Ji. (2018). Mass Spectrometry of Gangliosides. In: Sonnino, S., Prinetti, A. (eds) Gangliosides. Methods in Molecular Biology, vol 1804. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8552-4_9
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DOI: https://doi.org/10.1007/978-1-4939-8552-4_9
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