Abstract
Viral protein cage-based nanoreactors can be generated by encapsulation of catalytic metal nanoparticles within the capsid structure. In this method, coat proteins of the cowpea chlorotic mottle virus (CCMV) are used to sequester gold nanoparticles (Au NPs) in buffered solutions at neutral pH to form CCMV-Au hybrid nanoparticles. This chapter describes detailed methods for the encapsulation of Au NPs into CCMV protein cages. Protocols for the reduction of nitroarenes by using CCMV-Au NPs as catalyst are described as an example for the catalytic activity of Au NPs in the protein cages.
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References
Takale BS, Bao M, Yamamoto Y (2014) Gold nanoparticle (AuNPs) and gold nanopore (AuNPore) catalysts in organic synthesis. Org Biomol Chem 12:2005–2027
Chen Y, Qiu J, Wang X et al (2006) Preparation and application of highly dispersed gold nanoparticles supported on silica for catalytic hydrogenation of aromatic nitro compounds. J Catal 242:227–230
Wu S, Dzubiella J, Kaiser J et al (2012) Thermosensitive Au-PNIPA Yolk–Shell nanoparticles with tunable selectivity for catalysis. Angew Chem Int Ed 51:2229–2233
Comellas-Aragones M, Engelkamp H, Claessen VI et al (2007) A virus-based single-enzyme nanoreactor. Nat Nanotechnol 2:635–639
Bode SA, Minten IJ, Nolte RJM et al (2011) Reactions inside nanoscale protein cages. Nanoscale 3:2376–2389
Douglas T, Young M (1998) Host-guest encapsulation of materials by assembled virus protein cages. Nature 393:152–155
Wang T, Zhang Z, Gao D et al (2011) Encapsulation of gold nanoparticles by simian virus 40 capsids. Nanoscale 3:4275–4282
Jutz G, van Rijn P, Santos Miranda B et al (2015) Ferritin: a versatile building block for bionanotechnology. Chem Rev 115:1653–1701
Culver JN, Brown AD, Zang F et al (2015) Plant virus directed fabrication of nanoscale materials and devices. Virology 479–480:200–212
Bancroft JB, Hiebert E (1967) Formation of an infectious nucleoprotein from protein and nucleic acid isolated from a small spherical virus. Virology 32:354–356
Bancroft JB, Hills GJ, Markham R (1967) A study of the self-assembly process in a small spherical virus formation of organized structures from protein subunits in vitro. Virology 31:354–379
Speir JA, Munshi S, Wang G et al (1995) Structures of the native and swollen forms of cowpea chlorotic mottle virus determined by X-ray crystallography and cryo-electron microscopy. Structure 3:63–78
Kwak M, Minten IJ, Anaya D-M et al (2010) Virus-like particles templated by DNA micelles: a general method for loading virus nanocarriers. J Am Chem Soc 132:7834–7835
Brasch M, de la Escosura A, Ma Y et al (2011) Encapsulation of phthalocyanine supramolecular stacks into virus-like particles. J Am Chem Soc 133:6878–6881
Liu A, Verwegen M, de Ruiter MV et al (2016) Protein cages as containers for gold nanoparticles. J Phys Chem B 120:6352–6357
Liu A, Traulsen CHH, Cornelissen JJLM (2016) Nitroarene reduction by a virus protein cage based Nanoreactor. ACS Catal 6:3084–3091
Biondi I, Laurenczy G, Dyson PJ (2011) Synthesis of gold nanoparticle catalysts based on a new water-soluble ionic polymer. Inorg Chem 50:8038–8045
Gangula A, Podila R, M R, Karanam L et al (2011) Catalytic reduction of 4-Nitrophenol using biogenic gold and silver nanoparticles derived from Breynia rhamnoides. Langmuir 27:15268–15274
Acknowledgments
We acknowledge financial support from the research program of the Dutch Polymer Institute (DPI), Project #777t (DPI, P.O. Box 902, 5600 AX Eindhoven, the Netherlands) and ERC Consolidator Grant (Protcage#616907).
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Liu, A., de Ruiter, M.V., Maassen, S.J., Cornelissen, J.J.L.M. (2018). Nanoreactors via Encapsulation of Catalytic Gold Nanoparticles within Cowpea Chlorotic Mottle Virus Protein Cages. In: Udit, A. (eds) Protein Scaffolds. Methods in Molecular Biology, vol 1798. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7893-9_1
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DOI: https://doi.org/10.1007/978-1-4939-7893-9_1
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