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Chromatin Immunoprecipitation for Identification of Protein–DNA Interactions in Human Cells

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Two-Hybrid Systems

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1794))

Abstract

Chromatin immunoprecipitation (ChIP) is a powerful technique allowing for investigation of protein–DNA interactions in living cells. Here, we provide a detailed step-by-step protocol for ChIP and highlight important considerations, challenges and pitfalls often encountered in the ChIP procedure. Furthermore, we present data of key quality control (QC) steps and exemplify material performance validation on transcription factor ChIP to provide a QC guide for setting up ChIP. Finally, we provide guidelines for scaling of the ChIP procedure to ChIP sequencing (ChIP-seq) and discuss important considerations associated with this.

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Author notes

  1. Bjørk Ditlev Larsen and Martin Rønn Madsen contributed equally to this work.

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark

    Bjørk Ditlev Larsen, Martin Rønn Madsen, Ronni Nielsen & Susanne Mandrup

Authors
  1. Bjørk Ditlev Larsen
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  2. Martin Rønn Madsen
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  3. Ronni Nielsen
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  4. Susanne Mandrup
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Corresponding author

Correspondence to Susanne Mandrup .

Editor information

Editors and Affiliations

  1. Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM)–Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Montegancedo UPM, Madrid, Spain

    Luis Oñate-Sánchez

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Larsen, B.D., Madsen, M.R., Nielsen, R., Mandrup, S. (2018). Chromatin Immunoprecipitation for Identification of Protein–DNA Interactions in Human Cells. In: Oñate-Sánchez, L. (eds) Two-Hybrid Systems. Methods in Molecular Biology, vol 1794. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7871-7_24

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  • DOI: https://doi.org/10.1007/978-1-4939-7871-7_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7870-0

  • Online ISBN: 978-1-4939-7871-7

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