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Organotypic Slice Cultures to Study Oligodendrocyte Proliferation, Fate, and Myelination

  • Protocol
Myelin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1791))

Abstract

Oligodendrocyte development and myelination are processes in the central nervous system that are regulated by cell intrinsic and extrinsic mechanisms. Organotypic slice cultures provide a simple method for studying factors that affect oligodendrocyte proliferation, differentiation, and myelination in the context of the local cellular environment. Here we show that major glial cell types and neurons are preserved in slice cultures from postnatal mouse forebrain, and their morphological characteristics are retained. We further demonstrate that cellular processes requiring interactions with neighboring cells such as myelination can proceed in slice culture.

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Acknowledgments

This work was supported by grants from NIH (R01 NS073425 and R01 NS074870) to AN. We thank Youfen Sun for maintaining the transgenic mouse colony.

Author information

Authors and Affiliations

  1. Department of Biological Sciences, Dartmouth College, Hanover, NH, USA

    Robert A. Hill

  2. Department of Physiology and Neurobiology, University of Connecticut, Storrs, CT, USA

    Amin Sherafat, Robert A. Hill & Akiko Nishiyama

  3. Department of Neurology, Yale School of Medicine, New Haven, CT, USA

    Robert A. Hill

  4. Institute of Systems Genomics, University of Connecticut, Storrs, CT, USA

    Akiko Nishiyama

  5. Institute of Brain and Cognitive Science, University of Connecticut, Storrs, CT, USA

    Akiko Nishiyama

Authors
  1. Amin Sherafat
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  2. Robert A. Hill
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  3. Akiko Nishiyama
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Corresponding author

Correspondence to Akiko Nishiyama .

Editor information

Editors and Affiliations

  1. Nerve Disorders Laboratory, CIC bioGUNE, Derio, Vizcaya, Spain

    Ashwin Woodhoo

  2. Basque Foundation for Science, IKERBASQUE, Bilbao, Vizcaya, Spain

    Ashwin Woodhoo

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Sherafat, A., Hill, R.A., Nishiyama, A. (2018). Organotypic Slice Cultures to Study Oligodendrocyte Proliferation, Fate, and Myelination. In: Woodhoo, A. (eds) Myelin. Methods in Molecular Biology, vol 1791. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7862-5_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7862-5_11

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7861-8

  • Online ISBN: 978-1-4939-7862-5

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