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NMR-Based Prostate Cancer Metabolomics

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Prostate Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1786))

Abstract

Prostate cancer is the second most common malignancy, and the fifth leading cause of cancer-related death among men, worldwide. A major unsolved clinical challenge in prostate cancer is the ability to accurately distinguish indolent cancer types from the aggressive ones. Reprogramming of metabolism is now a widely accepted hallmark of cancer development, where cancer cells must be able to convert nutrients to biomass while maintaining energy production. Metabolomics is the large-scale study of small molecules, commonly known as metabolites, within cells, biofluids, tissues, or organisms. Nuclear magnetic resonance (NMR) spectroscopy is commonly applied in metabolomics studies of cancer. This chapter provides protocols for NMR-based metabolomics of cell cultures, biofluids (serum and urine), and intact tissue, with concurrent advice for optimal biobanking and sample preparation procedures.

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Author information

Authors and Affiliations

  1. Department of Circulation and Medical Imaging, NTNU - The Norwegian University of Science and Technology, Trondheim, Norway

    Leslie R. Euceda, Maria K. Andersen, May-Britt Tessem & Maria T. Grinde

  2. Department of Clinical and Molecular Medicine, NTNU - The Norwegian University of Science and Technology, Trondheim, Norway

    Siver A. Moestue

  3. Department of Pharmacy, Faculty of Health Sciences, Nord University, Bodø, Norway

    Siver A. Moestue

  4. Department of Circulation and Medical Imaging, NTNU - The Norwegian University of Science and Technology, Trondheim, Norway

    Tone F. Bathen

Authors
  1. Leslie R. Euceda
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  2. Maria K. Andersen
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  3. May-Britt Tessem
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  4. Siver A. Moestue
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  5. Maria T. Grinde
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  6. Tone F. Bathen
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Corresponding author

Correspondence to Tone F. Bathen .

Editor information

Editors and Affiliations

  1. Department of Urology, Innsbruch Medical University, Innsbruck, Austria

    Zoran Culig

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Euceda, L.R., Andersen, M.K., Tessem, MB., Moestue, S.A., Grinde, M.T., Bathen, T.F. (2018). NMR-Based Prostate Cancer Metabolomics. In: Culig, Z. (eds) Prostate Cancer. Methods in Molecular Biology, vol 1786. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7845-8_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7845-8_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7843-4

  • Online ISBN: 978-1-4939-7845-8

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