Abstract
Plants synthesize and emit a large range of volatile organic compounds (VOCs) that play important roles in their interactions with the environment, from attracting pollinators and seed dispersers to protectants such as repellants and pathogen inhibitors. As such, the development of techniques for headspace collection of volatiles in combination with gas chromatography–mass spectrometry (GC-MS) has an important impact on our understanding of the biosynthesis of plant VOCs. Furthermore, knowledge of the plant VOCs can be valuable in relation to plant breeding for improving fruit flavor or enhancing resistance to insects or pathogens. This chapter describes a reliable method for extracting volatile compounds by headspace solid-phase microextraction (HS-SPME), and separate and detect them by GC-MS.
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References
Pichersky E, Noel JP, Dudareva N (2006) Biosynthesis of plant volatiles: Nature’s diversity and ingenuity. Science 331:808–811
Pichersky E, Gershenzon J (2002) The formation and function of plant volatiles: Perfumes for pollinator attraction and defense. Curr Opin Plant Biol 5:237–243
Dudareva N, Pichersky E, Gershenzon J (2004) Biochemistry of plant volatiles. Plant Physiol 135:1893–1902
Linskens HF, Jackson JF (1997) Modern methods of plant analysis. plant volatile analysis. Springer, New York
Millar JG, Sims JJ (1998) Preparation, cleanup, and preliminary fractionation of extracts. In: Millar JG, Haynes KF (eds) Methods in chemical ecology. Kluwer Academic Publishers, Boston, pp 1–37
Rambla JL, López-Gresa MP, Bellés JM, Granell A (2015) Metabolomic profiling of plant tissue. In: Alonso JM, Stepanova AN (eds) Plant functional genomics, Methods in molecular biology. Springer, New York, pp 221–235
Flamini G, Cioni PL, Morelli I (2002) Differences in the fragrances of pollen and different floral parts of male and female flowers of Laurus nobilis. J Agric Chem 50:4647–4652
Holzinger R, Sandoval-Soto L, Rottenberger S et al (2000) Emissions of volatile organic compounds from Quercus ilex L. measured by proton-transfer-reaction mass spectrometry under different environmental conditions. J Geo- phys Res 105:20573–20579
Rohloff J, Bones AM (2005) Volatile profiling of Arabidopsis thaliana-Putative olfactory compounds in plant communications. Phytochemistry 66:1941–1955
Zorrilla-Fontanesi Y, Rambla JL, Cabeza A et al (2012) Genetic analysis of strawberry fruit aroma and identification of O-methyltransferase FaOMT as the locus controlling natural variation in mesifurano content. Plant Physiol 159:851–870
Sumner LW, Amberg A, Barrett D et al (2007) Proposed minimum reporting standards for chemical analysis Chemical Analysis Working Group (CAWG) Metabolomics Standards Initiative (MSI). Metabolomics 3:211–221
Fernie AR, Aharoni A, Willmitzer L et al (2011) Recommendations for reporting metabolite data. Plant Cell 23:2477–2482
Dunn WB, Erban A, Weber RJM et al (2013) Mass appeal: metabolite identification in mass spectrometry-focused untargeted metabolomics. Metabolomics 9:S44–S66
Rochat B (2017) Proposed confidence scale and ID score in the identification of known-unknown compounds using high resolution MS data. J Am Mass Spectrom 28:709–723
Strehmel N, Hummel J, Erban A et al (2008) Retention index thresholds for compound matching in GC-MS metabolite profiling. J Chromatogr B 871:182–190
Acknowledgments
We thank the Spanish Ministerio de Ciencia e Innovación, Ministerio de Economía y Competitividad-FEDER, University of Málaga through the Grants AGL2012-40066-CO2-02 and Ramón y Cajal program (RYC-09170). The authors also thank the European Union’s Horizon 2020 research and innovation programme (grant agreement number 679303) and the Max Planck Society for financial support.
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Vallarino, J.G., Erban, A., Fehrle, I., Fernie, A.R., Kopka, J., Osorio, S. (2018). Acquisition of Volatile Compounds by Gas Chromatography–Mass Spectrometry (GC-MS). In: António, C. (eds) Plant Metabolomics. Methods in Molecular Biology, vol 1778. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7819-9_16
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DOI: https://doi.org/10.1007/978-1-4939-7819-9_16
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