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Imaging Protein Fibers at the Nanoscale and In Situ

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Peptide Self-Assembly

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1777))

Abstract

Protein self-assembly offers a rich repertoire of tools and technologies. However, despite significant progress in this area, a deterministic measure of the phenomenon, which might lead to predictable relationships between protein components, assembly mechanisms, and ultimately function, is lacking. Often the challenge relates to the choice of the most informative and precise measurements that can link the chemistry of the building blocks with the resulting assembly, ideally in situ and in real time. Using the example of protein fibrillogenesis—a self-assembly process fundamental to nearly every aspect of biological organization, from viral assembly to tissue restoration—this chapter demonstrates how protein self-assembly can be visually and precisely measured while providing measurement protocols applicable to other self-assembly systems.

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Author information

Authors and Affiliations

  1. National Physical Laboratory, Middlesex, UK

    Angelo Bella, Michael Shaw, Emiliana De Santis & Maxim G. Ryadnov

Authors
  1. Angelo Bella
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  2. Michael Shaw
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  3. Emiliana De Santis
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  4. Maxim G. Ryadnov
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Corresponding author

Correspondence to Maxim G. Ryadnov .

Editor information

Editors and Affiliations

  1. Department of Chemistry, University of Rochester, Rochester, New York, USA

    Bradley L. Nilsson

  2. Department of Medicinal Chemistry, Institute for Translational Neuroscience, University of Minnesota, Minneapolis, Minnesota, USA

    Todd M. Doran

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Bella, A., Shaw, M., De Santis, E., Ryadnov, M.G. (2018). Imaging Protein Fibers at the Nanoscale and In Situ. In: Nilsson, B., Doran, T. (eds) Peptide Self-Assembly. Methods in Molecular Biology, vol 1777. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7811-3_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7811-3_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7809-0

  • Online ISBN: 978-1-4939-7811-3

  • eBook Packages: Springer Protocols

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