Abstract
Adipose tissue is as an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Here, we describe methods from our own laboratory and the literature for the isolation and expansion of adipose-derived stem cells (ASCs). We present a large scale procedure suitable for processing >100 mL volumes of lipoaspirate tissue specimens by collagenase digestion and a related procedure suitable for processing adipose tissue aspirates without digestion.
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Notes
- 1.
This period can vary from 24 to 72 h depending on the number of cells attached to the plastic surface (observed under microscope).
- 2.
Using ethanol, there is a risk that the lipids will be eluted from the cells.
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Li, J., Curley, J.L., Floyd, Z.E., Wu, X., Halvorsen, Y.D.C., Gimble, J.M. (2018). Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates. In: Bunnell, B.A., Gimble, J.M. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 1773. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7799-4_13
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DOI: https://doi.org/10.1007/978-1-4939-7799-4_13
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