Abstract
This chapter compares two different techniques for monitoring photosynthetic O2 production: the widespread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We describe how a simple membrane inlet for MIMS can be made out of a commercial Clark-type cell, and outline the advantages and drawbacks of the two techniques to guide researchers in deciding which method to use. Protocols and examples are given for measuring O2 evolution rates and for determining the number of chlorophyll molecules per active photosystem II reaction center.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Renger G, Hanssum B (2009) Oxygen detection in biological systems. Photosynth Res 102:487–498
Van Gorkom HJ, Gast P (1996) Measurement of photosynthetic oxygen evolution. In: Amesz J, Hoff AJ (eds) Biophysical techniques in photosynthesis, Advances in photosynthesis, vol 3. Kluwer Academic Publishers, Dordrecht, pp 391–405
Lakowicz JR (1999) Principles of fluorescence spectroscopy, 2nd edn. Kluwer Academic/Plenum Publishers, New York
Chodavarapu VP, Shubin DO, Bukowski RM et al (2007) CMOS-based phase fluorometric oxygen sensor system. IEEE Trans Circ Syst 54:111–118
Shevela D, Messinger J (2013) Studying the oxidation of water to molecular oxygen in photosynthetic and artificial systems by time-resolved membrane-inlet mass spectrometry. Front Plant Sci 4:473. https://doi.org/10.3389/fpls.2013.00473
Konermann L, Messinger J, Hillier W (2008) Mass spectrometry based methods for studying kinetics and dynamics in biological systems. In: Amesz J, Hoff AJ (eds) Biophysical techniques in photosynthesis, Series advances in photosynthesis and respiration, vol 26. Springer, Dordrecht, pp 167–190
Cheah MH, Millar AH, Myers RC et al (2014) Online oxygen kinetic isotope effects using membrane inlet mass spectrometry can differentiate between oxidases for mechanistic studies and calculation of their contributions to oxygen consumption in whole tissues. Anal Chem 86:5171–5178
Beckmann K, Messinger J, Badger MR et al (2009) On-line mass spectrometry: membrane inlet sampling. Photosynth Res 102:511–522
Delieu T, Walker DA (1972) An improved cathode for the measurement of photosynthetic oxygen evolution by isolated chloroplasts. New Phytol 71:201–255
Gonzalez L, Bolano C, Pellissier F (2001) Use of oxygen electrode in measurements of photosynthesis and respiration. In: Reiggosa Roger MJ (ed) Handbook of plant ecophysiology techniques. Kluwer Academic Publishers, Dordrecht, pp 141–153
Walker D (1987) The use of the oxygen electrode and fluorescence probes in simple measurements of photosynthesis. Oxygraphics Limited, Sheffield
Canvin DT, Berry JA, Badger MR et al (1980) Oxygen exchange in leaves in the light. Plant Physiol 66:302–307
Clark LC, Wolf R, Granger D et al (1953) Continuous recording of blood oxygen tensions by polarography. J Appl Physiol 6:189–193
Hoch G, Kok B (1963) A mass spectrometer inlet system for sampling gases dissolved in liquid phases. Arch Biochem Biophys 101:160–170
Johnson RC, Cooks RG, Allen TM et al (2000) Membrane introduction mass spectrometry: trends and applications. Mass Spectrom Rev 19:1–37
Silva ACB, Augusti R, Dalmazio I et al (1999) MIMS evaluation of pervaporation processes. Phys Chem Chem Phys 1:2501–2504
Carpentier R (ed) (2011) Photosynthesis research protocols, Methods in molecular biology, vol 684, 2nd edn. Springer, Clifton
Shevela D, Beckmann K, Clausen J et al (2011) Membrane-inlet mass spectrometry reveals a high driving force for oxygen production by photosystem II. Proc Natl Acad Sci U S A 108:3602–3607
Shevela D, Messinger J (2012) Probing the turnover efficiency of photosystem II membrane fragments with different electron acceptors. Biochim Biophys Acta 1817:1208–1212
Acknowledgments
We thank Dr. Mun Hon Cheah for careful reading of the manuscript and valuable suggestions. The Swedish Energy Agency (Energimyndigheten), Swedish Science Foundation (Vetenskapsrådet), and the K & A Wallenberg Foundation are acknowledged for financial support.
Author information
Authors and Affiliations
Corresponding authors
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Shevela, D., Schröder, W.P., Messinger, J. (2018). Liquid-Phase Measurements of Photosynthetic Oxygen Evolution. In: Covshoff, S. (eds) Photosynthesis. Methods in Molecular Biology, vol 1770. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7786-4_11
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7786-4_11
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7785-7
Online ISBN: 978-1-4939-7786-4
eBook Packages: Springer Protocols