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Spectral and Imaging Flow Cytometry in Phytoplankton Research

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Cellular Heterogeneity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1745))

Abstract

Spectral and imaging flow cytometry are emerging technologies that allow quantifying spectral, fluorescent, and/or morphological parameters of heterogeneous cellular populations. The protocol describes a detailed step-by-step analysis of microalgae using these techniques and examples from our laboratory (Aphanizomenon sp., Cryptomonas pyrenoidifera, and Chlorella sp.). Moreover, the chapter will be helpful to scientists who want to perform spectral flow cytometry and apply principal component analysis.

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Acknowledgments

Support for this work was provided by grants from MES of Republic of Kazakhstan #4350/ГФ4 and PI NURIS 055 project #100/14 to N.S.B. We are grateful to Steve Conway and Greg Veltri, Sony Biotechnology Inc., and John Daley, Dana-Farber Cancer Institute, for access to spectral flow cytometer and to Brian Hall and Richard De Marco (Amnis-Merck) for their advice. Veronika Dashkova, Ivan A. Vorobjev, and Natasha S. Barteneva contributed equally to this work.

Author information

Author notes

  1. Veronika Dashkova, Ivan A. Vorobjev, and Natasha S. Barteneva contributed equally to this work.

Authors and Affiliations

  1. National Laboratory Astana, Department of Biology, School of Science and Technology, Nazarbayev University, Astana, Kazakhstan

    Veronika Dashkova

  2. Sony Biotechnology Inc., San Jose, CA, USA

    Jeff Clapper

  3. PCMM-Harvard Medical School, Boston Children’s Hospital, Boston, MA, USA

    Natasha S. Barteneva

  4. Department of Biology, School of Sciences and Technology, Nazarbayev University, Astana, Kazakhstan

    Ivan A. Vorobjev & Natasha S. Barteneva

Authors
  1. Veronika Dashkova
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  2. Jeff Clapper
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  3. Ivan A. Vorobjev
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  4. Natasha S. Barteneva
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Corresponding authors

Correspondence to Ivan A. Vorobjev or Natasha S. Barteneva .

Editor information

Editors and Affiliations

  1. PCMM-Harvard Medical School, Boston Children’s Hospital, Boston, Massachusetts, USA

    Natasha S. Barteneva

  2. Department of Biology, School of Sciences and Technology, Nazarbayev University, Astana, Kazakhstan

    Ivan A. Vorobjev

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Dashkova, V., Clapper, J., Vorobjev, I.A., Barteneva, N.S. (2018). Spectral and Imaging Flow Cytometry in Phytoplankton Research. In: Barteneva, N., Vorobjev, I. (eds) Cellular Heterogeneity. Methods in Molecular Biology, vol 1745. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7680-5_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7680-5_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7679-9

  • Online ISBN: 978-1-4939-7680-5

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