Abstract
Biomaterials can be utilized to assist in the transplantation of Schwann cells to the central and peripheral nervous system. The biomaterials can be natural or man-made, and can have preformed shapes or injectable formats. Biomaterials can play multiple roles in cellular transplantation; for example, they can assist with cellular integration and protect Schwann cells from cell death initiated by the lack of a substrate, an occurrence known as “anoikis.” In addition, biomaterials can be engineered to increase cell proliferation and differentiation by the addition of ligands bound to the substrate. Here, we describe the incorporation of Schwann cells to both man-made and natural matrices for in vitro and in vivo measures relevant to Schwann cell transplantation strategies.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Schense JC, Bloch J, Aebischer P, Hubbell JA (2000) Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension. Nat Biotechnol 18(4):415–419. https://doi.org/10.1038/74473
Oudega M, Xu XM (2006) Schwann cell transplantation for repair of the adult spinal cord. J Neurotrauma 23(3-4):453–467. https://doi.org/10.1089/neu.2006.23.453
Chen A, Xu XM, Kleitman N, Bunge MB (1996) Methylprednisolone administration improves axonal regeneration into Schwann cell grafts in transected adult rat thoracic spinal cord. Exp Neurol 138(2):261–276. https://doi.org/10.1006/exnr.1996.0065
Oudega M, Xu XM, Guenard V, Kleitman N, Bunge MB (1997) A combination of insulin-like growth factor-I and platelet-derived growth factor enhances myelination but diminishes axonal regeneration into Schwann cell grafts in the adult rat spinal cord. Glia 19(3):247–258
Plant GW, Bates ML, Bunge MB (2001) Inhibitory proteoglycan immunoreactivity is higher at the caudal than the rostral Schwann cell graft-transected spinal cord interface. Mol Cell Neurosci 17(3):471–487. https://doi.org/10.1006/mcne.2000.0948
Xu XM, Chen A, Guenard V, Kleitman N, Bunge MB (1997) Bridging Schwann cell transplants promote axonal regeneration from both the rostral and caudal stumps of transected adult rat spinal cord. J Neurocytol 26(1):1–16
Xu XM, Guenard V, Kleitman N, Bunge MB (1995) Axonal regeneration into Schwann cell-seeded guidance channels grafted into transected adult rat spinal cord. J Comp Neurol 351(1):145–160. https://doi.org/10.1002/cne.903510113
Guenard V, Kleitman N, Morrissey TK, Bunge RP, Aebischer P (1992) Syngeneic Schwann cells derived from adult nerves seeded in semipermeable guidance channels enhance peripheral nerve regeneration. J Neurosci 12(9):3310–3320
Kromer LF, Cornbrooks CJ (1985) Transplants of Schwann cell cultures promote axonal regeneration in the adult mammalian brain. Proc Natl Acad Sci U S A 82(18):6330–6334
Chirila TV, Constable IJ, Crawford GJ, Vijayasekaran S, Thompson DE, Chen YC, Fletcher WA, Griffin BJ (1993) Poly(2-hydroxyethyl methacrylate) sponges as implant materials: in vivo and in vitro evaluation of cellular invasion. Biomaterials 14(1):26–38
Plant GW, Chirila TV, Harvey AR (1998) Implantation of collagen IV/poly(2-hydroxyethyl methacrylate) hydrogels containing Schwann cells into the lesioned rat optic tract. Cell Transplant 7(4):381–391
Plant GW, Harvey AR, Chirila TV (1995) Axonal growth within poly (2-hydroxyethyl methacrylate) sponges infiltrated with Schwann cells and implanted into the lesioned rat optic tract. Brain Res 671(1):119–130
Joosten EA, Bar PR, Gispen WH (1995) Collagen implants and cortico-spinal axonal growth after mid-thoracic spinal cord lesion in the adult rat. J Neurosci Res 41(4):481–490. https://doi.org/10.1002/jnr.490410407
Plant GW, Christensen CL, Oudega M, Bunge MB (2003) Delayed transplantation of olfactory ensheathing glia promotes sparing/regeneration of supraspinal axons in the contused adult rat spinal cord. J Neurotrauma 20(1):1–16. https://doi.org/10.1089/08977150360517146
Barbour HR, Plant CD, Harvey AR, Plant GW (2013) Tissue sparing, behavioral recovery, supraspinal axonal sparing/regeneration following sub-acute glial transplantation in a model of spinal cord contusion. BMC Neurosci 14:106. https://doi.org/10.1186/1471-2202-14-106
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Plant, C.D., Plant, G.W. (2018). Schwann Cell Transplantation Methods Using Biomaterials. In: Monje, P., Kim, H. (eds) Schwann Cells. Methods in Molecular Biology, vol 1739. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7649-2_29
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7649-2_29
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7648-5
Online ISBN: 978-1-4939-7649-2
eBook Packages: Springer Protocols