Abstract
We present a procedure to synthesize gold nanostars which are readily internalized by mouse hippocampal cells. These nanoparticles tend to localize close to the nuclei and have a surface plasmon resonance in the near infrared therefore we can stimulate their luminescence with two-photon technology while imaging structural or functional neuronal fluorescent markers. This allows us to evaluate the effect of the nanoparticles on the neuron’s behavior without any other external stimuli. We found that these nanoparticles increase the firing rate by modifying the activity of the potassium channels. In addition, by increasing the intensity of the laser used for imaging, we can stimulate the one-photon surface plasmon mode of the nanoparticles to destroy single cells and organelles containing nanostars while neighboring cells remain intact.
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Acknowledgments
This project was supported by the NIH/NIGMS MARC U*STAR GM07717, the National Institute on Minority Health and Health Disparities RCMI G12MD007591 from the National Institutes of Health and the NSF PREM DMR 0934218.
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Santamaria, F., Peralta, X.G. (2018). Two Applications of Gold Nanostars to Hippocampal Neuronal Cells: Localized Photothermal Ablation and Stimulation of Firing Rate. In: Santamaria, F., Peralta, X. (eds) Use of Nanoparticles in Neuroscience. Neuromethods, vol 135. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7584-6_6
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DOI: https://doi.org/10.1007/978-1-4939-7584-6_6
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