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GLUT Characterization Using Frog Xenopus laevis Oocytes

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Glucose Transport

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1713))

Abstract

Xenopus laevis oocytes are a useful heterologous expression system for expressing glucose transporters (GLUTs) and examining their functions. In this chapter, we provide a detailed protocol on oocyte extraction and preparation for GLUT9 protein expression. Furthermore, we describe the determination of GLUT9 overexpression level by biotinylation and Western blotting analysis. Finally, we also describe how GLUT9-expressing oocytes can be used to measure urate kinetics by radioisotopes as well as two-microelectrode voltage clamping techniques.

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Authors and Affiliations

  1. Membrane Protein Research Group, University of Alberta, 7-55 Medical Sciences Building, Edmonton, AB, Canada, T6G 2H7

    Wentong Long, Debbie O’Neill & Chris I. Cheeseman

Authors
  1. Wentong Long
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  2. Debbie O’Neill
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  3. Chris I. Cheeseman
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Corresponding author

Correspondence to Chris I. Cheeseman .

Editor information

Editors and Affiliations

  1. Department of Experimental Medical Science, Lund University, Lund, Sweden

    Karin Lindkvist-Petersson

  2. Department of Experimental Medical Science, Lund University, Lund, Sweden

    Jesper S Hansen

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Long, W., O’Neill, D., Cheeseman, C.I. (2018). GLUT Characterization Using Frog Xenopus laevis Oocytes. In: Lindkvist-Petersson, K., Hansen, J. (eds) Glucose Transport. Methods in Molecular Biology, vol 1713. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7507-5_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7507-5_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7506-8

  • Online ISBN: 978-1-4939-7507-5

  • eBook Packages: Springer Protocols

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