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Parallelized Antibody Selection in Microtiter Plates

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Phage Display

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1701))

Abstract

The most common in vitro technology to generate human antibodies is phage display. This technology is a key technology to select recombinant antibodies for the use as research tools, in diagnostic tests, and for the development of therapeutics.

In this review, the high-throughput compatible selection of antibodies (scFv) in microtiter plates is described. The given detailed protocols allow the antibody selection (“panning”), screening and identification of monoclonal antibodies in less than 1 week.

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Acknowledgments

This review is an updated and revised version of [9].

Author information

Authors and Affiliations

  1. Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Spielmannstr. 7, 38106, Braunschweig, Germany

    Giulio Russo, Doris Meier, Saskia Helmsing, Esther Wenzel & Fabian Oberle

  2. Abteilung Biotechnologie, Institut für Biochemie, Biotechnologie und Bioinformatik, Technische Universität Braunschweig, Braunschweig, Germany

    André Frenzel & Michael Hust

  3. YUMAB GmbH, Braunschweig, Germany

    André Frenzel

Authors
  1. Giulio Russo
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  2. Doris Meier
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  3. Saskia Helmsing
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  4. Esther Wenzel
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  5. Fabian Oberle
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  6. André Frenzel
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  7. Michael Hust
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Corresponding author

Correspondence to Michael Hust .

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Editors and Affiliations

  1. Technische Universität Braunschweig, Braunschweig, Germany

    Michael Hust

  2. Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden, Penang, Malaysia

    Theam Soon Lim

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Russo, G. et al. (2018). Parallelized Antibody Selection in Microtiter Plates. In: Hust, M., Lim, T. (eds) Phage Display. Methods in Molecular Biology, vol 1701. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7447-4_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7447-4_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7446-7

  • Online ISBN: 978-1-4939-7447-4

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