Abstract
Pancreatic ductal adenocarcinoma (PDAC) is the most predominant type of pancreatic cancer and presents one of the highest mortality rates when compared with other carcinomas. The absence of efficient treatment options for PDAC prompted us to investigate whether microRNA inhibition, combined or not with chemotherapeutic agents, would constitute a promising therapeutic approach for this disease. In this chapter, we describe several methods and procedures that can be used to evaluate the potential of new therapeutic strategies involving oligonucleotides against overexpressed microRNAs, in PDAC, either alone or in combination with low amounts of chemotherapeutic drugs.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Saif MW (2011) Pancreatic neoplasm in 2011 : an update. J Pancreas 12:316–321
Hidalgo M, Von Hoff DD (2012) Translational therapeutic opportunities in ductal adenocarcinoma of the pancreas. Clin Cancer Res 18:4249–4256
Siegel R, Naishadham D, Jemal A (2012) Cancer statistics, 2012. CA Cancer J Clin 62:10–29
Marco MDI, Cicilia RDI, Macchini M et al (2010) Metastatic pancreatic cancer : is gemcitabine still the best standard treatment ? (Review). Oncol Rep 23:1183–1192
Mardin WA, Mees ST (2009) MicroRNAs: novel diagnostic and therapeutic tools for pancreatic ductal adenocarcinoma? Ann Surg Oncol 16:3183–3189
Zhang Y, Li M, Wang H et al (2009) Profiling of 95 microRNAs in pancreatic cancer cell lines and surgical specimens by real-time PCR analysis. World J Surg 33:698–709
Szafranska E, Davison TS, John J et al (2007) MicroRNA expression alterations are linked to tumorigenesis and non-neoplastic processes in pancreatic ductal adenocarcinoma. Oncogene 26:4442–4452
Volinia S, Calin G, Liu CG et al (2006) A microRNA expression signature of human solid tumors defines cancer gene targets. Proc Natl Acad Sci U S A 103:2257–2261
Bloomston M, Frankel WL, Petrocca F et al (2007) MicroRNA expression patterns to differentiate pancreatic adenocarcinoma from normal pancreas and chronic pancreatitis. JAMA 297:1901–1908
Passadouro M, Pedroso de Lima MC, Faneca H (2014) MicroRNA modulation combined with sunitinib as a novel therapeutic strategy for pancreatic cancer. Int J Nanomedicine 9:3203–3217
Faneca H, Düzgünes N, Pedroso de Lima MC (2010) Fluorescence methods for evaluating lipoplex-mediated gene delivery. In: Weissig V (ed) Methods in molecular biology: liposomes, vol 606. Humana Press Inc., New Jersey, pp 425–437
Faneca H, Simões S, Pedroso de Lima MC (2004) Association of albumin or protamine to lipoplexes: enhancement of transfection and resistance to serum. J Gene Med 6:681–692
Fiske CH, Subbarow Y (1925) The colorimetric determination of phosphorus. J Biol Chem 66:375–400
Bartlett GR (1959) Phosphorus assay in column chromatography. J Biol Chem 234:466–468
Faneca H, Faustino A, Pedroso de Lima MC (2008) Synergistic antitumoral effect of non-viral HSV-Tk/GCV gene therapy and vinblastine in mammary adenocarcinoma cells. J Control Release 126:175–184
Lu J, Tsourkas A (2011) Quantification of miRNA abundance in single cells using locked nucleic acid-FISH and enzyme-labeled fluorescence. Methods Mol Biol 680:77–88
Düzgüneş N (2003) Preparation and quantitation of small unilamellar liposomes and large unilamellar reverse-phase evaporation liposomes. Methods Enzymol 367:23–27
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Passadouro, M., Faneca, H. (2018). Combination of Anti-miRNAs Oligonucleotides with Low Amounts of Chemotherapeutic Agents for Pancreatic Cancer Therapy. In: Wu, W. (eds) MicroRNA and Cancer. Methods in Molecular Biology, vol 1699. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7435-1_11
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7435-1_11
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7433-7
Online ISBN: 978-1-4939-7435-1
eBook Packages: Springer Protocols