Abstract
Plant ER membranes are the major site of biosynthesis of several lipid families (phospholipids, sphingolipids, neutral lipids such as sterols and triacylglycerols). The structural diversity of lipids presents considerable challenges to comprehensive lipid analysis. This chapter will briefly review the various biosynthetic pathways and will detail several aspects of the lipid analysis: lipid extraction, handling, separation, detection, identification, and data presentation. The different tools/approaches used for lipid analysis will also be discussed in relation to the studies to be carried out on lipid metabolism and function.
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References
Morré DJ, Panel C, Morré DM, Sandelius AS, Moreau P, Andersson B (1991) Cell-free transfer and sorting of membrane lipids in spinach. Donor and acceptor specificity. Protoplasma 160:49–64
Parsons HT, Drakakaki G, Heazlewood JL (2013) Proteomic dissection of the Arabidopsis Golgi and trans-Golgi network. Front Plant Sci 3:298
Parsons HT, Fernández-Niño SM, Heazlewood JL (2014) Separation of the plant Golgi apparatus and endoplasmic reticulum by free-flow electrophoresis. Methods Mol Biol 1072:527–539
Li-Beisson Y, Shorrosh B, Beisson F, Andersson MX, Arondel V, Bates PD, Baud S, Bird D, DeBono A, Durrett TP, Franke RB, Graham IA, Katayama K, Kelly AA, Larson T, Markham JE, Miquel M, Molina I, Nishida I, Rowland O, Samuels L, Schmid KM, Wada H, Welti R, Xu C, Zallot R, Ohlrogge J (2013) Acyl-lipid metabolism. Arabidopsis Book 11:e0161
Folch J, Lees M, Stanley GHS (1957) A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 226:497–509
Heape AM, Juguelin H, Boiron F, Cassagne C (1985) Improved one dimensional thin layer chromatographic technique for polar lipids. J Chromatogr 332:391–395
Macala LJ, Yo RK, Ando S (1983) Analysis of brain lipids by high performance TLC and densitometry. J Lipid Res 24:1243–1250
Laloi M, Perret AM, Chatre L, Melser S, Cantrel C, Vaultier MN, Zachowski A, Bathany K, Schmitter JM, Vallet M, Lessire R, Hartmann MA, Moreau P (2007) Insights into the role of specific lipids in the formation and delivery of lipid microdomains to the plasma membrane of plant cells. Plant Physiol 143:461–472
Cacas JL, Melser S, Domergue F, Joubès J, Bourdenx B, Schmitter JM, Mongrand S (2012) Rapid nanoscale quantitative analysis of plant sphingolipid long-chain bases by GC-MS. Anal Bioanal Chem 403:2745–2755
Hillig I, Leipelt M, Ott C, Zahringer U, Warnecke D, Heinz E (2003) Formation of glucosylceramides and sterol glucoside by a UDP-glucose-dependent glucosylceramide synthase from cotton expressed in Pichia pastoris. FEBS Lett 553:365–369
Juguelin H, Heape MA, Boiron F, Cassagne C (1986) A quantitative developmental study of neutral lipids during myelinogenesis in the peripheral nervous system of normal and trembler mice. Brain Res 390:249–252
Brown DJ, Dupont FM (1989) Lipid composition of plasma membranes and endomembranes prepared from roots of barley (Hordeum vulgare L.): effects of salt. Plant Physiol 90:955–961
Grindstaff KK, Fielding LA, Brodl MR (1996) Effect of gibberellin and heat shock on the lipid composition of endoplasmic reticulum in barley aleurone layers. Plant Physiol 110:571–581
Moreau P, Bessoule JJ, Mongrand S, Testet E, Vincent P, Cassagne C (1998) Lipid trafficking in plant cells. Progr Lipid Res 37:371–391
Acknowledgments
Metabolome Facility-MetaboHUB (ANR-11-INBS-0010) of the Functional Genomic Center of Bordeaux.
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Fouillen, L., Maneta-Peyret, L., Moreau, P. (2018). ER Membrane Lipid Composition and Metabolism: Lipidomic Analysis. In: Hawes, C., Kriechbaumer, V. (eds) The Plant Endoplasmic Reticulum . Methods in Molecular Biology, vol 1691. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7389-7_10
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DOI: https://doi.org/10.1007/978-1-4939-7389-7_10
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