Abstract
ChIP-seq is the current method of choice for genome-wide protein location analysis. Here, we present a native (non-cross-linked) ChIP procedure suitable for histone proteins, coupled with an efficient library preparation technique for subsequent next-generation sequencing. The method enables ChIP-seq starting with 50,000 or more cells.
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Acknowledgment
G.D.G. is funded by the Norwegian South-Eastern Regional Health Authority. T.R. was funded by the EU-FP7 project EpiTrain (REA grant agreement n° 316758). We would also like to thank Diagenode for their support.
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Ribarska, T., Gilfillan, G.D. (2018). Native Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) from Low Cell Numbers. In: Visa, N., Jordán-Pla, A. (eds) Chromatin Immunoprecipitation. Methods in Molecular Biology, vol 1689. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7380-4_14
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DOI: https://doi.org/10.1007/978-1-4939-7380-4_14
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7379-8
Online ISBN: 978-1-4939-7380-4
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