Abstract
Chromatin immunoprecipitation is used to measure the binding of transcription factors to target DNA sequences in order to better understand transcriptional regulation. Here, we describe a process to analyze bacterial transcription factor binding in the context of an infected eukaryotic host cell. Using this approach, we measured the binding kinetics of three Chlamydia trachomatis transcription factors within infected cells, and demonstrated temporal changes in binding.
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References
Collas P (2010) The current state of chromatin immunoprecipitation. Mol Biotechnol 45:87–100
Hanson BR, Tan M (2015) Transcriptional regulation of the Chlamydia heat shock stress response in an intracellular infection. Mol Microbiol 97:1158–1167
Hanson BR, Tan M (2016) Intra-ChIP: studying gene regulation in an intracellular pathogen. Curr Genet 62:547–551
AbdelRahman YM, Belland RJ (2005) The chlamydial developmental cycle. FEMS Microbiol Rev 29:949–959
Belland RJ, Zhong G, Crane DD, Hogan D, Sturdevant D, Sharma J, Beatty WL, Caldwell HD (2003) Genomic transcriptional profiling of the developmental cycle of Chlamydia trachomatis. Proc Natl Acad Sci U S A 100:8478–8483
Peirson SN, Butler JN, Foster RG (2003) Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis. Nucleic Acids Res 31:e73
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Hanson, B.R., Tan, M. (2018). Using Intra-ChIP to Measure Protein–DNA Interactions in Intracellular Pathogens. In: Visa, N., Jordán-Pla, A. (eds) Chromatin Immunoprecipitation. Methods in Molecular Biology, vol 1689. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7380-4_13
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DOI: https://doi.org/10.1007/978-1-4939-7380-4_13
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7379-8
Online ISBN: 978-1-4939-7380-4
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