Abstract
Cellular quiescence is a key component of hematopoietic stem cell (HSC) homeostasis; therefore, a reliable method to measure HSC cell division is critical in many studies. However, measuring the proliferation rate of largely quiescent and rare populations of cells can be challenging. Bromo-deoxyuridine (BrdU) incorporation into replicating DNA is a commonly used and highly reproducible method to detect cell division history. Here, we describe a protocol for BrdU incorporation analysis in hematopoietic stem and progenitor cells that can provide a sensitive measure of cell division even in rare cell populations. In combination with flow cytometry, this method can be generalized to analyze other cell populations and other tissues as identified by cell surface markers.
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Acknowledgments
The work summarized here was supported by grants from the NIDDK DK060445 (KAM) and the NHLBI K08HL098898, the Department of Defense IDEA award in bone marrow failure research (10505346), the Caroline Wiess Law Foundation for Molecular Medicine, the Aplastic Anemia and MDS International Foundation Liviya Anderson Award, and a March of Dimes Basil O’Connor Starter Scholar Award (KYK).
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Matatall, K.A., Kadmon, C.S., King, K.Y. (2018). Detecting Hematopoietic Stem Cell Proliferation Using BrdU Incorporation. In: Lacorazza, H. (eds) Cellular Quiescence. Methods in Molecular Biology, vol 1686. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7371-2_7
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DOI: https://doi.org/10.1007/978-1-4939-7371-2_7
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