Abstract
Bacterial contamination can confound the results of in vitro and in vivo preclinical tests. This protocol describes a procedure for detection of microbial contamination in nanotechnology-based formulations. Nanoparticle samples and controls are spread on the surface of agar and growth of bacterial colonies is monitored after 72 h of incubation. The intended purpose of this assay is to avoid introduction of microbial contamination into in vitro cell cultures and in vivo animal studies utilizing the test nanomaterial. This assay is not intended to certify the material as sterile.
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References
USP standard 51 (2007) Antimicrobial effectiveness testing USP 30 NF 25 1
USP standard 61 (2007) Microbial limit tests USP 30 NF 25 1
USP standard 71 (2007) Sterility tests USP 30 NF 25 1
Acknowledgment
This project has been funded in whole or in part with Federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. HHSN261200800001E. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. Government.
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Potter, T.M., Neun, B.W., Ilinskaya, A.N., Dobrovolskaia, M.A. (2018). Detection of Bacterial Contamination in Nanoparticle Formulations by Agar Plate Test. In: McNeil, S. (eds) Characterization of Nanoparticles Intended for Drug Delivery. Methods in Molecular Biology, vol 1682. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7352-1_2
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DOI: https://doi.org/10.1007/978-1-4939-7352-1_2
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7350-7
Online ISBN: 978-1-4939-7352-1
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