Abstract
Transcription is the initial and generally the most sensitive step to cellular needs and environmental cues. Thus, it serves as a major mechanism controlling gene expression. Using reverse-transcription quantitative polymerase chain reaction technology (RT-qPCR), we will present how to quantify the transcriptional expression of NMDARs subunits during brain development and in both healthy and pathological conditions.
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Acknowledgments
This work was supported by INSERM (Institut National de la Santé et de la Recherche Médicale) and by the European Union Seventh Framework Programme FP7/2007–2013 under the project DESIRE (grant agreement no. 602531).
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Pallesi-Pocachard, E. (2017). Quantification of NMDAR Subunit Genes Expression by qRT-PCR. In: Burnashev, N., Szepetowski, P. (eds) NMDA Receptors. Methods in Molecular Biology, vol 1677. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7321-7_2
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DOI: https://doi.org/10.1007/978-1-4939-7321-7_2
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