Abstract
Coagulation factor VIII (FVIII) is an important glycoprotein involved in the extrinsic coagulation cascade. Mutations in FVIII gene results in hemophilia A, a recessive coagulation disorder that is clinically managed by administration of purified FVIII from blood donors or recombinant FVIII. Because of its fundamental therapeutic application, biotechnological production of FVIII requires rigid quality control and monitoring in patients and clinical trials. Here, we describe a protocol for a mass spectrometry based approach termed selective reaction monitoring (SRM) as an important alternative tool for accurate and sensitive quantitation of purified or recombinant FVIII.
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Acknowledgments
This research was supported by FAPESP (Young Scientist Grants—Proc. No. 2011/09347-1), Center for Cell Based Thereapy—CTC-CEPID (Proc. FAPESP 2013/08135-2) and CISBi-NAP. G.P.L. received a fellowship from CNPq Proc. No. 130142/2015-8, and V.M.F. received a fellowship from CNPq, Proc. No. 308561/2014-7.
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do Nascimento Filho, E.G., Lanfredi, G.P., Abreu-Neto, M.S., Covas, D.T., Faça, V.M. (2018). Quantification of Coagulation Factor VIII by Selective Reaction Monitoring. In: Picanço-Castro, V., Swiech, K. (eds) Recombinant Glycoprotein Production. Methods in Molecular Biology, vol 1674. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7312-5_22
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DOI: https://doi.org/10.1007/978-1-4939-7312-5_22
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7312-5
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