Abstract
Breeding of date palm is complicated because of its long life cycle and heterozygous nature. Sexual propagation of date palm does not produce true-to-type plants. Sex of date palms cannot be identified until the first flowering stage. Molecular markers such as random amplified polymorphic DNA (RAPD), sequence-characterized amplified regions (SCAR), and simple sequence repeats (SSR) have successfully been used to identify the sex-linked loci in the plant genome and to isolate the corresponding genes. This chapter highlights the use of three molecular markers including RAPD, SCAR, and SSR to identify the gender of date palm seedlings.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Maryam JMJ, Awan FS, Ahmad S, Khan IA (2016) Development of molecular method for sex identification in date palm (Phoenix dactylifera L) plantlets using novel sex-linked microsatellite markers 3. Biotech 6:22. doi:10.1007/s13205-015-0321-6
Siljak-Yakovlev S, Cerbah M, Sarr A, Benmalek S, Bounaga N, Coba dela pena T, Brown SC (1996) Chromosomal sex determination and heterochromatin structure in date palm. Sex Plant Reprod 9:127–132
Bawa KS (1980) Evolution of dioecy in flowering plants. Annu Rev Ecol Syst 11:15–39
Dellaporta SL, Calderon-Urrea A (1993) Sex determination in flowering plants. Plant Cell 5:1241–1251
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–11
Aberlenc-Bertossi F, Daher A, Chabrillange N, Tregear JW, Mohamed N (2011) Sex determination in date palm: new perspectives on an old theme. Plant and animal genomes XIX conference, W519: sex chromosomes and sex determination. Town and Country Convention Center, San Diego, CA. pp 15–19
Daher A, Adam H, Chabrillange N, Collin M, Mohamed N, Tregear JW, Aberlenc-Bertossi F (2010) Cell cycle arrest characterizes the transition from a bisexual floral bud to a unisexual flower in Phoenix dactylifera. Ann Bot 106:255–266
Torres AM, Tisserat B (1980) Leaf isozymes as genetic markers in date palms. Amer J Bot 67:162–167
Majourhat K, Bendiab K, Medraoui L, Baaziz M (2002) Diversity of leaf peroxidases in date palm (Phoenix dactylifera L.) as revealed in an example of marginal (seedling derived) palm groves. Sci Hortic 95:31–38
Qacif N, Baaziz M, Bendiab K (2007) Biochemical investigations on peroxidase contents of male and female inflorescences of date palm (Phoenix dactylifera L.) Sci Hortic 114:298–301
Biffi R, Restivo FM, Tassi F, Carboni A, Marziani GP, Sapda A, Falvigna A (1995) A restriction fragment length polymorphism probe for early diagnosis of gender in Asparagus officinalis L. Hortic Sci 30:1463–1464
Hormaza J, Dollo L, Polito V (1994) Identification of a RAPD marker linked to sex determination in Pistacia vera using bulked segregant analysis. Theor Appl Genet 89:9–13
Welsh J, McClelland M (1990) Fingerprinting genome-using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218
Ben Abdallah A, Sitti K, Leporive P, Du Jardin P (2000) Identification de cultivars de palmier dattier (Phoenix dactylifera L.) par l’amplification aleatoire d’ADN (RAPD). Cah Agric 9:103–107
Trifi M, Rhouma A, Marrakchi M (2000) Phylogenetic relationships in Tunisian date-palms (Phoenix dactylifera L.) germplasm collection using DNA amplification fingerprinting. Agronomie 20:665–671
Paran I, Michelmore RW (1993) Development of reliable PCR based markers linked to downy mildew resistance in lettuce. Theor Appl Genet 85:985–993
Elmeer K, Mattat I (2012) Marker-assisted sex differentiation in date palm using simple sequence repeats. Biotech 2:241–247
Acknowledgment
This research work was funded by the Higher Education Commission, Pakistan, and supported by Plant Functional Genomics Lab., Centre of Agricultural Biochemistry and Biotechnology (CABB), and Plant Tissue Culture Cell, University of Agriculture Faisalabad, Pakistan.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Awan, F.S., Maryam, Jaskani, M.J., Sadia, B. (2017). Gender Identification in Date Palm Using Molecular Markers. In: Al-Khayri, J., Jain, S., Johnson, D. (eds) Date Palm Biotechnology Protocols Volume II. Methods in Molecular Biology, vol 1638. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7159-6_18
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7159-6_18
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7158-9
Online ISBN: 978-1-4939-7159-6
eBook Packages: Springer Protocols