Abstract
Circulating tumor cells (CTCs) are disseminated tumor cells that reflect the tumors of origin and can provide a liquid biopsy that would potentially enable noninvasive tumor profiling, treatment monitoring, and identification of targeted treatments. Accurate and rapid purification of CTCs holds great potential to improve cancer care but the task remains technically challenging. Microfluidic isolation of CTCs within microscale vortices enables high-throughput and size-based purification of rare CTCs from bodily fluids. Collected cells are highly pure, viable, and easily accessible, allowing seamless integration with various downstream applications. Here, we describe how to fabricate the High-Throughput Vortex Chip (Vortex-HT) and to process diluted whole blood for CTC collection. Lastly, immunostaining and imaging protocols for CTC classification and corresponding CTC image galleries are reported.
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Acknowledgement
The authors thank all patients and healthy blood donors for contributions to these studies. Samples were collected from the UCLA Hematology and Oncology Santa Monica Clinic and Stanford Medical Center under institutional review board approved protocols (UCLA IRB#11-001798 and Stanford IRB#5630). The work is supported by a sponsored research agreement from Vortex Biosciences, Inc. The Regents of the University of California and all authors have financial interests in Vortex Biosciences, Inc.
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Hur, S.C., Che, J., Di Carlo, D. (2017). Microscale Laminar Vortices for High-Purity Extraction and Release of Circulating Tumor Cells. In: M. Magbanua, M., W. Park, J. (eds) Circulating Tumor Cells. Methods in Molecular Biology, vol 1634. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7144-2_5
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DOI: https://doi.org/10.1007/978-1-4939-7144-2_5
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