Abstract
Historically, the diagnosis and classification of acute leukemia involved morphologic review of blasts in the peripheral blood and bone marrow smears and cytochemical staining. Cytochemical stains, which are enzymatic colorimetric reactions that occur in the cells of interest, were necessary to assign and confirm myeloid and lymphoid lineage. In the current WHO 2008 Classification of leukemia, immunophenotyping and cytogenetic analysis have largely replaced cytochemical staining in the characterization of acute leukemias. Nonetheless, cytochemical testing remains a useful adjunct assay for the proper classification of acute leukemia in a number of diagnostic settings. This chapter reviews the principles of the most common cytochemical stains, their procedures and guides to interpretation, and results in acute myeloid leukemia.
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References
Jaffe ES, Vardiman JW, Campo E et al (2011) Hematopathology. Saunders/Elsevier, Philadelphia, PA
Swerdlow SH, Harris NL, Jaffe ES et al (2008) WHO classification of tumours of haematopoietic and lymphoid tissue. IARC, Lyon
O'Donnell MR, Abboud CN, Altman J et al (2012) Acute myeloid leukemia. J Natl Compr Cancer Netw 10(8):984–1021
Alvarnas JC, Brown PA, Aoun P et al (2012) Acute lymphoblastic leukemia. J Natl Compr Cancer Netw 10(7):858–914
Ley TJ, Ding L, Raphael BJ et al (2013) Genomic and epigenomic landscapes of adult de novo acute myeloid leukemia. N Engl J Med 368(22):2059–2074. doi:10.1056/NEJMoa1301689
Altman AJ (1985) Cytologic diagnosis of the acute nonlymphoid leukemias. I. Morphologic, cytochemical, and ultrastructural features. Am J Pediatr Hematol Oncol 7(1):21–44
Arber DA, Snyder DS, Fine M et al (2001) Myeloperoxidase immunoreactivity in adult acute lymphoblastic leukemia. Am J Clin Pathol 116(1):25–33. doi:10.1309/hdue-dn2e-b72e-jk99
Mhawech P, Buffone GJ, Khan SP et al (2001) Cytochemical staining and flow cytometry methods applied to the diagnosis of acute leukemia in the pediatric population: an assessment of relative usefulness. J Pediatr Hematol Oncol 23(2):89–92
Yam LT, Li CY, Crosby WH (1971) Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol 55(3):283–290
Li CY, Lam KW, Yam LT (1973) Esterases in human leukocytes. J Histochem Cytochem 21(1):1–12
Hanker JS, Yates PE, Metz CB et al (1977) A new specific, sensitive and non-carcinogenic reagent for the demonstration of horseradish peroxidase. Histochem J 9(6):789–792
Resnitzky P, Shaft D, Yaari A et al (1994) Distinct intracellular lysozyme content in normal granulocytes and monocytes: a quantitative immunoperoxidase and ultrastructural immunogold study. J Histochem Cytochem 42(11):1471–1477
Seo IS, Li CY, Yam LT (1993) Myelodysplastic syndrome: diagnostic implications of cytochemical and immunocytochemical studies. Mayo Clin Proc 68(1):47–53
Lanza F, Latorraca A, Musto P et al (1991) Cytochemically unreactive neutrophils from subjects with myeloperoxidase (mpo) deficiency show a complex pattern of immunoreactivity with anti-mpo monoclonal antibodies: a flow cytometric and immunocytochemical study. Ann Hematol 63(2):94–100
Horobin RW (1981) Structure-staining relationships in histochemistry and biological staining. Part 3: some comments on the intentional and artifactual staining of lipids. Acta Histochem Suppl 24:237–246
Grande M (1977) Monocytic differentiation of cluster cells in agar culture of normal human bone marrow. A cytochemical investigation. Biomedicine 26(5):326–330
Behm FG (1990) Morphologic and cytochemical characteristics of childhood lymphoblastic leukemia. Hematol Oncol Clin North Am 4(4):715–741
Kass L (1979) Cytochemistry of esterases. CRC Crit Rev Clin Lab Sci 10(2):205–223
Elghetany MT, MacCallum JM, Davey FR (1990) The use of cytochemical procedures in the diagnosis and management of acute and chronic myeloid leukemia. Clin Lab Med 10(4):707–720
Pombo De Oliveira MS, Gregory C, Matutes E et al (1987) Cytochemical profile of megakaryoblastic leukaemia: a study with cytochemical methods, monoclonal antibodies, and ultrastructural cytochemistry. J Clin Pathol 40(6):663–669
Rodak BF, Fritsma GA, Keohane E (2011) Hematology: clinical principles and applications, 4th edn. Saunders, Philadelphia, PA
Catovsky D, Costello C (1979) Cytochemistry of normal and leukaemic lymphocytes: a review. Basic Appl Histochem 23(4):255–270
Srivastava BI, Rossowski W, Minowada J (1983) Cytochemical comparison of immunologically characterized human leukaemia/lymphoma cell lines representing different levels of maturation. Br J Cancer 47(6):771–779
Turgeon M (2004) Clinical hematology: theory and procedures, 4th edn. Lippincott Williams & Wilkins, Philadelphia, PA USA
Hayhoe FG, Quagliono D, Flemans RJ (1960) Consecutive use of Romanowsky and periodic-acid-Schiff techniques in the study of blood and bone-marrow cells. Br J Haematol 6:23–25
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Paessler, M.E., Helfrich, M., Wertheim, G.B.W. (2017). Cytochemical Staining. In: Fortina, P., Londin, E., Park, J., Kricka, L. (eds) Acute Myeloid Leukemia. Methods in Molecular Biology, vol 1633. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7142-8_2
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DOI: https://doi.org/10.1007/978-1-4939-7142-8_2
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