Abstract
The effective delivery of small interfering RNA (siRNA) to tumor cells remains a challenge for applications in cancer therapy. The development of polymeric nanoparticles with high siRNA loading efficacy has shown great potential for cancer targets. Double emulsion solvent evaporation technique is a useful tool for encapsulation of hydrophilic molecules (e.g., siRNA). Here we describe a versatile platform for siRNA delivery based on PLGA-PEG–cationic lipid nanoparticles by using the double emulsion method. The resulting nanoparticles show high encapsulation efficiency for siRNA (up to 90%) and demonstrate effective downregulation of the target genes in vitro and vivo.
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Wang, L., Griffel, B., Xu, X. (2017). Synthesis of PLGA–Lipid Hybrid Nanoparticles for siRNA Delivery Using the Emulsion Method PLGA-PEG–Lipid Nanoparticles for siRNA Delivery. In: Bindewald, E., Shapiro, B. (eds) RNA Nanostructures . Methods in Molecular Biology, vol 1632. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7138-1_15
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DOI: https://doi.org/10.1007/978-1-4939-7138-1_15
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7138-1
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