Abstract
Serological methods such as the enzyme-linked immunosorbent assay (ELISA) and virus neutralization test are fundamental tools used in diagnosis, seroepidemiological studies of filovirus transmission/prevalence, and the evaluation of vaccine immunogenicity and potential therapeutic antibodies. Filoviruses have a single transmembrane glycoprotein (GP), which is the only known target of neutralizing antibodies. Here we describe serological methods to quantify filovirus GP-specific antibodies.
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Acknowledgments
This work was supported by the Agency for Medical Research and Development (AMED) and Japan International Cooperation Agency (JICA) within the framework of the Science and Technology Research Partnership for Sustainable Development (SATREPS), the Japan Initiative for Global Research Network on Infectious Diseases (J-GRID), and a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology (MEXT).
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Furuyama, W., Miyamoto, H., Yoshida, R., Takada, A. (2017). Quantification of Filovirus Glycoprotein-Specific Antibodies. In: Hoenen, T., Groseth, A. (eds) Ebolaviruses. Methods in Molecular Biology, vol 1628. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7116-9_25
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DOI: https://doi.org/10.1007/978-1-4939-7116-9_25
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