Abstract
The conventional treatment for fungal diseases usually shows long periods of therapy and the high frequency of relapses and sequels. New strategies of the treatment are necessary. We have shown that the Mycobacterium leprae HSP65 gene can be successfully used as therapy against murine Paracoccidioidomycosis (PCM). Here, we described the methodology of DNAhsp65 immunotherapy in mice infected with the dimorphic fungus Paracoccidioides brasiliensis, one of PCM agent, evaluating cytokines levels, fungal burden, and lung injury. Our results provide a new prospective on the immunotherapy of mycosis.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Bolhassani A, Rafati S (2008) Heat-shock proteins as powerful weapons in vaccine development. Expert Rev Vaccines 7(8):1185–1199
Fioretti D, Iurescia S, Fazio VM et al (2010) DNA vaccines: developing new strategies against cancer. J Biomed Biotechnol 2010:174378
Wang XY, Kaneko Y, Repasky E et al (2000) Heat shock proteins and cancer immunotherapy. Immunol Investig 29(2):131–137
Doimo NT, Zárate-Bladés CR, Rodrigues RF et al (2014) Immunotherapy of tuberculosis with Mycobacterium leprae Hsp65 as a DNA vaccine triggers cross-reactive antibodies against mammalian Hsp60 but not pathological autoimmunity. Hum Vaccin Immunother 10(5):1238–1243
Lowrie DB (2006) DNA vaccines for therapy of tubercu losis: where are we now? Vaccine 24:1983–1989
Kumar A, Samant M (2016) DNA vaccine against visceral leishmaniasis: a promising approach for prevention and control. Parasite Immunol 38(5):273–281
Ribeiro AM, Bocca AL, Amaral AC et al (2010) HSP65 DNA as therapeutic strategy to treat experimental paracoccidioidomycosis. Vaccine 28(6):1528–1534
Siqueira IM, Ribeiro AM, Nóbrega YK et al (2013) DNA-hsp65 vaccine as therapeutic strategy to treat experimental chromoblastomycosis caused by Fonsecaea pedrosoi. Mycopathologia 175(5–6):463–475
Restrepo A (1985) The ecology of Paracoccidioides brasiliensis: a puzzle still unsolved. J Med Vet Mycol 23:323–334
Bocca AL, Amaral AC, Teixeira MM et al (2013) Paracoccidioidomycosis: eco-epidemiology, taxonomy and clinical and therapeutic issues. Future Microbiol 8(9):1177–1191
Rosada RS, de la Torre LG, Frantz FG et al (2008) Protection against tuberculosis by a single intranasal administration of DNA-hsp65 vaccine complexed with cationic liposomes. BMC Immunol 9:38
Singer-Vermes LM, Ciavaglia MC, Kashino SS et al (1992) The source of the growth-promoting factor affects the plating efficiency of Paracoccidioides brasiliensis. J Med Vet Mycol 30(3):261–264
Figueiredo F, Alves LMC, Silva CL (1993) Tumor necrosis factor production in vivo and in vitro in response to Paracoccidioides brasiliensis and the cell wall fractions thereof. Clin Exp Immunol 93:189
Green LC, Tannenbaum SR, Goldman P (1981) Nitrate synthesis in the germfree and conventional rat. Science 212:56–58
Dias MF, Mesquita J, Rodrigues N et al (2004) Viability of yeast form cells of Paracoccidioides brasiliensis after sonication. Med Mycol 42(1):43–49
Bocca AL, Hayashi EE, Pinheiro AG et al (1998) Treatment of Paracoccidioides brasiliensis-infected mice with a nitric oxide inhibitor prevents the failure of cell-mediated immune response. J Immunol 161(6):3056–3063
Schmidt HH, Wilke P, Evers B et al (1989) Enzymatic formation of nitrogen oxides from L-arginine in bovine brain cytosol. Biochem Biophys Res Commun 165(1):284–291
Acknowledgment
This work was supported by a grant from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Apoio à Pesquisa do Distrito Federal (FAPDF), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Ribeiro, A.M., Amaral, A.C., Felipe, M.S.S., Bocca, A.L. (2017). DNAhsp65 Vaccine as Therapy against Paracoccidioidomycosis. In: Kalkum, M., Semis, M. (eds) Vaccines for Invasive Fungal Infections. Methods in Molecular Biology, vol 1625. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7104-6_7
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7104-6_7
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7103-9
Online ISBN: 978-1-4939-7104-6
eBook Packages: Springer Protocols