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Bronchoalveolar Lavage: Quantitative Mass Spectrometry-Based Proteomics Analysis in Lung Diseases

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Serum/Plasma Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1619))

Abstract

Bronchoalveolar lavage (BAL) fluid, obtained by a relatively noninvasive procedure, is used as a practice for diagnosis of various lung diseases as source of cells for cytology analysis. The acellular component of BAL potentially can complement and be a key for the establishment of diagnostic or as a prognostic indicator. This chapter discusses the aspects of standardization of BAL sample preparation and processing and its implications on the BAL fluid proteome quantitative analysis by high-throughput mass spectrometry. The detailed conditions for quantitative analysis of BAL proteome in the context of biomarker discovery are introduced.

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Acknowledgment

All experiments including MS analysis were supported by Fundação para a Ciência e Tecnologia project EXPL/DTP-PIC/0616/2013. RM is supported by FCT Investigator Program 2012 (IF/01002/2012). A.S.C. is supported by grant SFRH/BPD/85569/2012 funded by Fundação para a Ciência e Tecnologia.

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Authors and Affiliations

  1. Computational and Experimental Biology Group, CEDOC—Chronic Diseases Research Center, Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Rua Câmara Pestana nº 6, 6-A, Lisboa, 1150-082, Portugal

    Ana Sofia Carvalho & Rune Matthiesen

Authors
  1. Ana Sofia Carvalho
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  2. Rune Matthiesen
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Corresponding author

Correspondence to Ana Sofia Carvalho .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia

    David W. Greening

  2. Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia

    Richard J. Simpson

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Carvalho, A.S., Matthiesen, R. (2017). Bronchoalveolar Lavage: Quantitative Mass Spectrometry-Based Proteomics Analysis in Lung Diseases. In: Greening, D., Simpson, R. (eds) Serum/Plasma Proteomics. Methods in Molecular Biology, vol 1619. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7057-5_34

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  • DOI: https://doi.org/10.1007/978-1-4939-7057-5_34

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7056-8

  • Online ISBN: 978-1-4939-7057-5

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