Abstract
Clinical lipidomics is an emerging biomarker discovery approach that compares lipid profiles under pathologically and physiologically normal conditions. Here we describe a method for the absolute (molar) quantification of more than 200 molecules from 14 major lipid classes from 5 μL of human blood plasma using high-resolution top-down shotgun mass spectrometry. Because of its technical simplicity and robustness, the protocol lends itself for high-throughput clinical lipidomics screens.
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Sales, S., Knittelfelder, O., Shevchenko, A. (2017). Lipidomics of Human Blood Plasma by High-Resolution Shotgun Mass Spectrometry. In: Greening, D., Simpson, R. (eds) Serum/Plasma Proteomics. Methods in Molecular Biology, vol 1619. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7057-5_16
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DOI: https://doi.org/10.1007/978-1-4939-7057-5_16
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