Abstract
Stem cell therapy for neurological disorders reached a pivotal point when the efficacy of several cell types was demonstrated in small-animal models. Translation of stem cell therapy is contingent upon overcoming the challenge of effective cell delivery to the human brain, which has a volume of ~1000 times larger than that of the mouse. Intra-arterial (IA) injection can achieve a broad, global, but if needed also spatially targeted biodistribution; however, its utility has been limited by unpredictable cell destination and homing as dictated by the vascular territory, as well as by safety concerns.
We show here that high-speed MRI can be used to visualize the intravascular distribution of a superparamagnetic iron oxide contrast agent and can thus be used to accurately predict the distribution of IA administered stem cells. Moreover, high-speed MRI enables the real-time visualization of cell homing, providing the opportunity for immediate intervention in the case of undesired biodistribution.
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Walczak, P., Janowski, M. (2017). Real-Time Dual MRI for Predicting and Subsequent Validation of Intra-Arterial Stem Cell Delivery to the Central Nervous System. In: Srivastava, A., Snyder, E., Teng, Y. (eds) Stem Cell Technologies in Neuroscience. Neuromethods, vol 126. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7024-7_12
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DOI: https://doi.org/10.1007/978-1-4939-7024-7_12
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