Abstract
Time-resolved macromolecular crystallography unifies protein structure determination with chemical kinetics. With the advent of fourth generation X-ray sources the time-resolution can be on the order of 10–40 fs, which opens the ultrafast time scale to structure determination. Fundamental motions and transitions associated with chemical reactions in proteins can now be observed. Moreover, new experimental approaches at synchrotrons allow for the straightforward investigation of all kind of reactions in biological macromolecules. Here, recent developments in the field are reviewed.
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Acknowledgment
M.S. thanks Vukica Šrajer for reading, and commenting on, an earlier version of the manuscript. This work is supported by the BioXFEL Science and Technology Center (NSF grant 1231306).
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Schmidt, M. (2017). Time-Resolved Macromolecular Crystallography at Modern X-Ray Sources. In: Wlodawer, A., Dauter, Z., Jaskolski, M. (eds) Protein Crystallography. Methods in Molecular Biology, vol 1607. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7000-1_11
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DOI: https://doi.org/10.1007/978-1-4939-7000-1_11
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