Abstract
Metabolite profiling allows for the identification of metabolites that become limiting during cell culture and/or for finding bottlenecks in metabolic pathways that limit culture growth and proliferation. Here we describe one protocol with two different sampling methodologies for GC–MS-based metabolite profiling. We also highlight an example of the types of datasets that are attainable and how such datasets can be evaluated to identify factors related to cell viability. We also demonstrate, via the same methodology, the accurate quantification of a number of metabolites of interest.
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Acknowledgment
We gratefully acknowledge the financial support of UK BBSRC and TSB. We are also grateful to Dr. Chris Sellick from MedImmune, who was instrumental in developing the methodology for this GC–MS-based technique.
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Evie, I.M., Dickson, A.J., Elvin, M. (2017). Metabolite Profiling of Mammalian Cell Culture Processes to Evaluate Cellular Viability. In: Gilbert, D., Friedrich, O. (eds) Cell Viability Assays. Methods in Molecular Biology, vol 1601. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6960-9_12
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DOI: https://doi.org/10.1007/978-1-4939-6960-9_12
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