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Microfluidic “Pouch” Chips for Immunoassays and Nucleic Acid Amplification Tests

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Biosensors and Biodetection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1572))

Abstract

Microfluidic cassettes (“chips”) for processing and analysis of clinical specimens and other sample types facilitate point-of-care (POC) immunoassays and nucleic acid based amplification tests. These single-use test chips can be self-contained and made amenable to autonomous operation—reducing or eliminating supporting instrumentation—by incorporating laminated, pliable “pouch” and membrane structures for fluid storage, pumping, mixing, and flow control. Materials and methods for integrating flexible pouch compartments and diaphragm valves into hard plastic (e.g., acrylic and polycarbonate) microfluidic “chips” for reagent storage, fluid actuation, and flow control are described. We review several versions of these pouch chips for immunoassay and nucleic acid amplification tests, and describe related fabrication techniques. These protocols thus offer a “toolbox” of methods for storage, pumping, and flow control functions in microfluidic devices.

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Author information

Authors and Affiliations

  1. School of Engineering and Applied Sciences, University of Pennsylvania, 220 S 33rd Street, Philadelphia, PA, 19104, USA

    Michael G. Mauk, Changchun Liu, Dafeng Chen, Jinzhao Song & Haim H. Bau

  2. Beijing University of Chemical Technology, Beijing, China

    Xianbo Qiu

Authors
  1. Michael G. Mauk
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  2. Changchun Liu
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  3. Xianbo Qiu
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  4. Dafeng Chen
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  5. Jinzhao Song
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  6. Haim H. Bau
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Corresponding author

Correspondence to Michael G. Mauk .

Editor information

Editors and Affiliations

  1. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Ben Prickril

  2. National Cancer Institute, National Institutes of Health, Rockville, Maryland, USA

    Avraham Rasooly

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Mauk, M.G., Liu, C., Qiu, X., Chen, D., Song, J., Bau, H.H. (2017). Microfluidic “Pouch” Chips for Immunoassays and Nucleic Acid Amplification Tests. In: Prickril, B., Rasooly, A. (eds) Biosensors and Biodetection. Methods in Molecular Biology, vol 1572. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6911-1_30

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  • DOI: https://doi.org/10.1007/978-1-4939-6911-1_30

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6910-4

  • Online ISBN: 978-1-4939-6911-1

  • eBook Packages: Springer Protocols

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