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Sterol Analysis by Quantitative Mass Spectrometry

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Cholesterol Homeostasis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1583))

Abstract

Analysis of sterols by mass spectrometry is a fundamental technique allowing for both qualitative and quantitative characterization of sterol molecular lipid species. Lipids are isolated from matrix or matrices by homogenization and solvent extraction, and converted into species amenable for ionization either by derivatization or adduct formation. Chromatogaphy (either gas or liquid phase) can assist with the resolution of sterols. Tandem mass spectrometry allows the precise identification of sterol lipid species, while comparison to internal standards added during extraction enables accurate quantification.

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References

  1. Sharpe LJ, Burns V, Brown AJ (2014) A lipidomic perspective on intermediates in cholesterol synthesis as indicators of disease status. J Genet Genomics 41(5):275–282

    Article  CAS  PubMed  Google Scholar 

  2. Martín MG, Pfrieger F, Dotti CG (2014) Cholesterol in brain disease: sometimes determinant and frequently implicated. EMBO Rep 15(10):1036–1052

    Article  PubMed  PubMed Central  Google Scholar 

  3. Poli G, Biasi F, Leonarduzzi G (2013) Oxysterols in the pathogenesis of major chronic diseases. Redox Biol 1(1):125–130

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Iuliano L, Micheletta F, Natoli S, Ginanni Corradini S, Iappelli M, Elisei W et al (2003) Measurement of oxysterols and α-tocopherol in plasma and tissue samples as indices of oxidant stress status. Anal Biochem 312(2):217–223

    Article  CAS  PubMed  Google Scholar 

  5. Griffiths WJ, Crick PJ, Wang Y (2013) Methods for oxysterol analysis: past, present and future. Biochem Pharmacol 86(1):3–14

    Article  CAS  PubMed  Google Scholar 

  6. Liebisch G, Binder M, Schifferer R, Langmann T, Schulz B, Schmitz G (2006) High throughput quantification of cholesterol and cholesteryl ester by electrospray ionization tandem mass spectrometry (ESI-MS/MS). BBA-Mol Cell Biol L 1761(1):121–128

    CAS  Google Scholar 

  7. Brown SH, Kunnen CM, Duchoslav E, Dolla NK, Kelso MJ, Papas EB et al (2013) A comparison of patient matched meibum and tear lipidomes. Invest Ophthalmol Vis Sci 54(12):7417–7424

    Article  CAS  PubMed  Google Scholar 

  8. van de Bovenkamp P, Kosmeijer-Schuil TG, Katan MB (1988) Quantification of oxysterols in Dutch foods: egg products and mixed diets. Lipids 23(11):1079–1085

    Article  PubMed  Google Scholar 

  9. Lee CYJ, Huang SH, Jenner AM, Halliwell B (2008) Measurement of F2-isoprostanes, hydroxyeicosatetraenoic products, and oxysterols from a single plasma sample. Free Radic Biol Med 44(7):1314–1322

    Article  CAS  PubMed  Google Scholar 

  10. Quehenberger O, Armando AM, Brown AH, Milne SB, Myers DS, Merrill AH et al (2010) Lipidomics reveals a remarkable diversity of lipids in human plasma. J Lipid Res 51(11):3299–3305

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Author information

Authors and Affiliations

  1. Bioanalytical Mass Spectrometry Facility, Mark Wainwright Analytical Centre, University of New South Wales, Sydney, NSW, Australia

    Andrew M. Jenner Ph.D.

  2. School of Biology and Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW, Australia

    Andrew M. Jenner Ph.D. & Simon H. J. Brown Ph.D.

Authors
  1. Andrew M. Jenner Ph.D.
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  2. Simon H. J. Brown Ph.D.
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Corresponding authors

Correspondence to Andrew M. Jenner Ph.D. or Simon H. J. Brown Ph.D. .

Editor information

Editors and Affiliations

  1. Faculty of Pharmacy, The University of Sydney, Sydney, New South Wales, Australia

    Ingrid C. Gelissen

  2. School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, New South Wales, Australia

    Andrew J. Brown

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Jenner, A.M., Brown, S.H.J. (2017). Sterol Analysis by Quantitative Mass Spectrometry. In: Gelissen, I., Brown, A. (eds) Cholesterol Homeostasis. Methods in Molecular Biology, vol 1583. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6875-6_17

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  • DOI: https://doi.org/10.1007/978-1-4939-6875-6_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6873-2

  • Online ISBN: 978-1-4939-6875-6

  • eBook Packages: Springer Protocols

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