Abstract
Mesenchymal stem cells (MSCs) are blossoming as a credible source for regenerative medical applications. The use of fetal MSCs is gaining momentum for therapeutic use. The ease of isolation, enhanced characteristics, and immunomodulation properties renders the utilization of fetal MSCs for numerous clinical applications. In this article, we will demonstrate a step-by-step protocol for isolation of Wharton’s jelly MSCs (WJMSCs) from the human umbilical cord matrix, preparation of human platelet lysate, fabricating amniotic membrane scaffold and mice model to study skin regeneration using a combination of MSCs and decellularized amniotic membrane scaffold.
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Acknowledgments
We are grateful to Department of Biotechnology (DBT), Government of India for Ramalingaswami Fellowship and research support grants (DBT Grants BT/PR8527/MED/31/234/2013; BT/PR8742/AGR/36/773/2013; and BT/PR 15420/MED/31/122/2011) to Sanjay Kumar. We would like to thank University Grants Commission (UGC), New Delhi, India for JRF/SRF fellowship to Balasubramanian Sundaram. We would like to thank Dr. Anne George Cherian, Department of Obstetrics & Gynaecology and Saranya Ravi for valuable assistance during this study.
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Sabapathy, V., Sundaram, B., Kumar, S. (2017). Therapeutic Application of Human Wharton Jelly Mesenchymal Stem Cells in Skin Injury of SCID. In: Di Nardo, P., Dhingra, S., Singla, D. (eds) Adult Stem Cells. Methods in Molecular Biology, vol 1553. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6756-8_9
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DOI: https://doi.org/10.1007/978-1-4939-6756-8_9
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6754-4
Online ISBN: 978-1-4939-6756-8
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