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Derivation of Mesenchymal Stem Cells from Embryonic Stem Cells: A Non-Variable and Inexhaustive Source of Adult Stem Cells

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Adult Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1553))

Abstract

Mesenchymal stem cells (MSCs) provide an opportunity to bring the field of regenerative medicine to realization. A lot of clinical trials are presently trying to establish their applicability in real-world scenarios. Some of the biggest challenges encountered in bringing MSCs from bench to bedside are the number of MSCs required, their procurement from various sources, and the batch-to-batch variability. This often leads to inconclusive results within and between different studies. Therefore, we have hereby proposed a simple protocol to source mesenchymal stem cells through differentiation of embryonic stem cells.

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Correspondence to Sanjiv Dhingra .

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Sequiera, G.L., Sareen, N., El-Rub, E.A., Dhingra, S. (2017). Derivation of Mesenchymal Stem Cells from Embryonic Stem Cells: A Non-Variable and Inexhaustive Source of Adult Stem Cells. In: Di Nardo, P., Dhingra, S., Singla, D. (eds) Adult Stem Cells. Methods in Molecular Biology, vol 1553. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6756-8_2

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  • DOI: https://doi.org/10.1007/978-1-4939-6756-8_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6754-4

  • Online ISBN: 978-1-4939-6756-8

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