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Establishment of EBV-Infected Lymphoblastoid Cell Lines

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Epstein Barr Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1532))

Abstract

Lymphoblastoid cell lines (LCLs) can be generated easily by in vitro EBV infection of B lymphocytes collected from any individual. In vitro, these EBV-infected B cell cultures yield proliferating, transformed lines referred to as lymphoblastoid cell lines (LCLs).

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References

  1. Pope JH, Horne MK, Scott W (1968) Transformation of foetal human leukocytes in vitro by filtrates of a human leukaemic cell line containing herpeslike virus. Int J Cancer 3:857–866

    Article  CAS  PubMed  Google Scholar 

  2. Rickinson AB, Kieff E (2001) Epstein–Barr virus. In: Knipe DM, Howley PM (eds) Fields virology, vol 2, 4th edn. Lippincott Williams and Wilkins, Philadelphia, pp 2575–2627

    Google Scholar 

  3. Thorley-Lawson DA (2001) Epstein-Barr virus: exploiting the immune system. Nat Rev Immunol 1:75–82

    Article  CAS  PubMed  Google Scholar 

  4. Kang MS, Kieff E (2015) Epstein–Barr virus latent genes. Exp Mol Med 47:e131

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Kieff E, Rickinson AB (2001) Epstein-Barr virus and its replication. In: Knipe DM, Howley PM (eds) Fields virology, vol 2, 4th edn. Lippincott Williams and Wilkins, Philadelphia, pp 2511–2574

    Google Scholar 

  6. Bird AG, McLachlan SM, Britton S (1981) Cyclosporin A promotes spontaneous outgrowth in vitro of Epstein-Barr virus-induced B-cell lines. Nature 289:300–301

    Article  CAS  PubMed  Google Scholar 

  7. Miller G, Lipman M (1973) Release of infectious Epstein-Barr virus by transformed marmoset leukocytes. Proc Natl Acad Sci U S A 70:190–194

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Daibata M, Humphreys RE, Takada K, Sairenji T (1990) Activation of latent EBV via anti-IgG-triggered, second messenger pathways in the Burkitt’s lymphoma cell line Akata. J Immunol 144:4788–4793

    CAS  PubMed  Google Scholar 

  9. Delecluse HJ, Feederle R, Behrends U, Mautner J (2008) Contribution of viral recombinants to the study of the immune response against the Epstein-Barr virus. Semin Cancer Biol 18:409–415

    Article  CAS  PubMed  Google Scholar 

  10. Rickinson AB, Rowe M, Hart IJ, Yao QY, Henderson LE et al (1984) T-cell-mediated regression of spontaneous and of Epstein-Barr virus-induced B-cell transformation in vitro: studies with cyclosporin A. Cell Immunol 87:646–658

    Article  CAS  PubMed  Google Scholar 

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Correspondence to Noemi Nagy .

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Nagy, N. (2017). Establishment of EBV-Infected Lymphoblastoid Cell Lines. In: Minarovits, J., Niller, H. (eds) Epstein Barr Virus. Methods in Molecular Biology, vol 1532. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6655-4_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6655-4_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6653-0

  • Online ISBN: 978-1-4939-6655-4

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