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Histone Purification from Saccharomyces cerevisiae

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Histones

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1528))

Abstract

The nucleosome structure consists of a histone octamer made by a tetramer of H3-H4 histones and two dimers of H2A-H2B. Nucleosomes undergo extensive posttranslational modifications that regulate nucleosome interactions, position, and stability.

We describe a protocol allowing the robust purification of histones from the yeast Saccharomyces cerevisiae. This method appears to be suitable to quantitatively analyze specific posttranslational histone modifications.

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References

  1. Dehé PM, Pamblanco M, Luciano P, Lebrun R, Moinier D, Sendra R, Verreault A, Tordera V, Géli V (2005) Histone H3 lysine 4 mono-methylation does not require ubiquitination of histone H2B. J Mol Biol 353:477–484

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Acknowledgments

We thank Vicente Tordera and Merce Pamblanco (University of Valencia) who showed us how to purify histones from S. cerevisiae.

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Authors and Affiliations

  1. Cancer Research Center of Marseille (CRCM), U1068 Inserm, UMR7258 CNRS, Aix Marseille University (AMU), Institut Paoli-Calmettes, Marseille, 13009, France

    Frederic Jourquin & Vincent Géli

Authors
  1. Frederic Jourquin
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  2. Vincent Géli
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Corresponding author

Correspondence to Vincent Géli .

Editor information

Editors and Affiliations

  1. Université de Sherbrooke Department of Biology, Sherbrooke, Québec, Canada

    Benoit Guillemette

  2. Department of Biology, Université de Sherbrooke, Sherbrooke, Québec, Canada

    Luc R. Gaudreau

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Cite this protocol

Jourquin, F., Géli, V. (2017). Histone Purification from Saccharomyces cerevisiae . In: Guillemette, B., Gaudreau, L. (eds) Histones. Methods in Molecular Biology, vol 1528. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6630-1_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6630-1_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6628-8

  • Online ISBN: 978-1-4939-6630-1

  • eBook Packages: Springer Protocols

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