Abstract
Here we describe an initial characterization of Campylobacter jejuni bacteriophages by host range analysis, genome size determination by pulsed-field gel electrophoresis, and receptor-type identification by screening mutants for phage sensitivity.
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References
Kropinski AM, Mazzocco A, Waddell TE, et al. (2009) Enumeration of bacteriophages by double agar overlay plaque assay. In: Clokie MRJ, Kropinski AM (eds) Bacteriophages: methods and protocols, Volume 1: Isolation, characterization, and interactions. p 69
Janež N, Kokošin A, Zaletel E et al (2014) Identification and characterization of new Campylobacter group III phages of animal origin. FEMS Microbiol Lett 359:64–71
Hansen VM, Rosenquist H, Baggesen DL et al (2007) Characterization of Campylobacter phages including analysis of host range by selected Campylobacter Penner serotypes. BMC Microbiol 18(7):90
El-Shibiny A, Connerton PL, Connerton IF (2005) Enumeration and diversity of campylobacters and bacteriophages isolated during the rearing cycles of free-range and organic chickens. Appl Environ Microbiol 71:1259–1266
Sørensen MCH, Gencay YE, Birk T et al (2015) Primary isolation strain determines both phage type and receptors recognised by Campylobacter jejuni bacteriophages. PLoS One 10:e0116287
Baldvinsson SB, Sørensen MCH, Vegge CS et al (2014) Campylobacter jejuni motility is required for infection of the flagellotropic bacteriophage F341. Appl Environ Microbiol 80:7096–7106
Holst Sørensen MC, van Alphen LB, Fodor C et al (2012) Phase variable expression of capsular polysaccharide modifications allows Campylobacter jejuni to avoid bacteriophage infection in chickens. Front Cell Infect Microbiol 2:11
Sørensen MCH, van Alphen LB, Harboe A, Li J et al (2011) Bacteriophage F336 recognizes the capsular phosphoramidate modification of Campylobacter jejuni NCTC11168. J Bacteriol 193:6742–6749
Coward C, Grant AJ, Swift C et al (2006) Phase-variable surface structures are required for infection of Campylobacter jejuni by bacteriophages. Appl Environ Microbiol 72:4638–4647
Brathwaite KJ, Siringan P, Moreton J et al (2013) Complete genome sequence of universal bacteriophage host strain Campylobacter jejuni subsp. jejuni PT14. Genome Announc 1:e00969–13
Lingohr E, Frost S, Johnson RP. (2009) Determination of bacteriophage genome size by pulsed-field gel electrophoresis. In: Clokie MRJ, Kropinski AM (eds) Bacteriophages: methods and protocols, Volume 2: Molecular and applied aspects. p 19
Javed MA, Ackermann HW, Azeredo J et al (2014) A suggested classification for two groups of Campylobacter myoviruses. Arch Virol 159:181–190
Ackermann HW (2009) Basic phage electron microscopy. In Clokie MRJ, Kropinski AM (eds) Bacteriophages: methods and protocols, Volume 1: Isolation, characterization, and interactions. p 113
Green MR, Sambrook J (2012) Molecular cloning: a laboratory manual, 4th edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
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Sørensen, M.C.H., Gencay, Y.E., Brøndsted, L. (2017). Methods for Initial Characterization of Campylobacter jejuni Bacteriophages. In: Butcher, J., Stintzi, A. (eds) Campylobacter jejuni. Methods in Molecular Biology, vol 1512. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6536-6_9
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DOI: https://doi.org/10.1007/978-1-4939-6536-6_9
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