Abstract
One of the most noticeable changes in T-cell immunity with aging is the expansion of memory CD8+ T cells, with a decline in naïve phenotype T cells that reflects both diminished thymopoiesis and the effects of chronic antigenic stimulation with age. Flow cytometry is a useful tool in evaluating immune cells including the phenotype characteristics of different T-cell subsets. Here, we show flow cytometric methods measuring the different subsets of human CD8+ T cells that change with aging.
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References
Reiner SL (2007) Development in motion: helper T cells at work. Cell 129:33–36
Lee N, Shin MS, Kang I (2012) T-cell biology in aging, with a focus on lung disease. J Gerontol A Biol Sci Med Sci 67:254–263
Starr TK, Jameson SC, Hogquist KA (2003) Positive and negative selection of T cells. Annu Rev Immunol 21:139–176
Murphy KM, Stockinger B (2010) Effector T cell plasticity: flexibility in the face of changing circumstances. Nat Immunol 11:674–680
Cui W, Kaech SM (2010) Generation of effector CD8+ T cells and their conversion to memory T cells. Immunol Rev 236:151–166
Shin MS, Lee N, Kang I (2011) Effector T-cell subsets in systemic lupus erythematosus: update focusing on Th17 cells. Curr Opin Rheumatol 23:444–448
Hong MS, Dan JM, Choi JY et al (2004) Age-associated changes in the frequency of naive, memory and effector CD8+ T cells. Mech Ageing Dev 125:615–618
Lee WW, Shin MS, Kang Y et al (2012) The relationship of cytomegalovirus (CMV) infection with circulatory IFN-alpha levels and IL-7 receptor alpha expression on CD8(+) T cells in human aging. Cytokine 58:332–335
Sallusto F, Lenig D, Forster R et al (1999) Two subsets of memory T lymphocytes with distinct homing potentials and effector functions. Nature 401:708–712
Kim HR, Hong MS, Dan JM et al (2006) Altered IL-7R{alpha} expression with aging and the potential implications of IL-7 therapy on CD8+ T-cell immune responses. Blood 107:2855–2862
Soloski MJ, Chrest FJ (2013) Multi-parameter flow cytometry for discovery of disease mechanisms in rheumatic diseases. Arthritis Rheum 65:1148–1156
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Shin, M.S., Kang, I. (2015). Analyzing the Effect of Aging on CD8+ T-Cell Phenotype Using Flow Cytometry. In: Shaw, A. (eds) Immunosenescence. Methods in Molecular Biology, vol 1343. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2963-4_10
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DOI: https://doi.org/10.1007/978-1-4939-2963-4_10
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2962-7
Online ISBN: 978-1-4939-2963-4
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