Abstract
In recent years, cell-based therapies using adult stem cells have attracted considerable interest in regenerative medicine. A tissue-engineered construct for cartilage repair should provide a support for the cell and allow sustained in situ delivery of bioactive factors capable of inducing cell differentiation into chondrocytes. Pharmacologically active microcarriers (PAMs), made of biodegradable and biocompatible poly (d,l-lactide-co-glycolide acid) (PLGA), are a unique system which combines these properties in an adaptable and simple microdevice. This device relies on nanoprecipitation of proteins encapsulated in polymeric microspheres with a solid in oil in water emulsion-solvent evaporation process, and their subsequent coating with extracellular matrix protein molecules. Here, we describe their preparation process, and some of their characterization methods for an application in cartilage tissue engineering.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Tatard VM, Sindji L, Branton J, Aubert-Pouëssel A, Colleau J, Benoit JP, Montero-Menei CN (2007) Pharmacologically active microcarriers releasing glial cell line – derived neurotrophic factor: survival and differentiation of embryonic dopaminergic neurons after grafting in hemiparkinsonian rats. Biomaterials 28:1978–1988
Tatard VM, Venier-Julienne MC, Benoit JP, Menei P, Montero-Menei CN (2004) In vivo evaluation of pharmacologically active microcarriers releasing nerve growth factor and conveying PC12 cells. Cell Transplant 13:573–583, Published online Epub 2004 (10.3727/000000004783983675)
Delcroix GJR, Garbayo E, Sindji L, Thomas O, Vanpouille-Box C, Schiller PC, Montero-Menei CN (2011) The therapeutic potential of human multipotent mesenchymal stromal cells combined with pharmacologically active microcarriers transplanted in hemi-parkinsonian rats. Biomaterials 32:1560–1573, Published online EpubFeb (10.1016/j.biomaterials.2010.10.041
Garbayo E, Curtis K, Raval A, DellaMorte D, Gomez LA, D'Ippolito G, Reiner T, Perez-Stable C, Howard G, Perez-Pinzon M, Montero-Menei C, Schiller P (2011) Neuroprotective properties of Marrow-Isolated Adult Multilineage-Inducible (MIAMI) cells in rat hippocampus following global cerebral ischemia are enhanced when complexed to biomimetic microcarriers. Xenotransplantation 18:286–286, Published online EpubSep-Oct
Morille M, Tran V-T, Garric X, Cayon J, Coudane J, Noel D, Venier-Julienne M-C, Montero-Menei CN (2013) New PLGA-P188-PLGA matrix enhances TGF-beta 3 release from pharmacologically active microcarriers and promotes chondrogenesis of mesenchymal stem cells. J Control Release 170:99–110, Published, online EpubAug 28
Tesseur I, Zou K, Berber E, Zhang H, Wyss-Coray T (2006) Highly sensitive and specific bioassay for measuring bioactive TGF-beta. BMC Cell Biol 7:15
Penna C et al (2013) Pharmacologically active microcarriers influence VEGF-A effects on mesenchymal stem cell survival. J Cell Mol Med 17:192–204
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Morille, M., Venier-Julienne, MC., Montero-Menei, C.N. (2015). Scaffolds for Controlled Release of Cartilage Growth Factors. In: Doran, P. (eds) Cartilage Tissue Engineering. Methods in Molecular Biology, vol 1340. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2938-2_12
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2938-2_12
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2937-5
Online ISBN: 978-1-4939-2938-2
eBook Packages: Springer Protocols