Skip to main content

Antiangiogenic Metargidin Peptide (AMEP) Gene Therapy in Disseminated Melanoma

  • Protocol
Gene Therapy of Solid Cancers

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1317))

Abstract

Gene delivery by electroporation is an efficient method for transfecting genes into various tissues including tumors. Here we present the treatment protocol used in a phase 1 study on gene electrotransfer of plasmid DNA encoding an antiangiogenic peptide into cutaneous melanoma.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Gothelf A, Gehl J (2010) Gene electrotransfer to skin; review of existing literature and clinical perspectives. Curr Gene Ther 10(4):287–299

    Article  CAS  PubMed  Google Scholar 

  2. Heller LC, Heller R (2006) In vivo electroporation for gene therapy. Hum Gene Ther 17(9):890–897

    Article  CAS  PubMed  Google Scholar 

  3. Mir LM, Moller PH, Andre F et al (2005) Electric pulse-mediated gene delivery to various animal tissues. Adv Genet 54:83–114

    CAS  PubMed  Google Scholar 

  4. Heller LC, Heller R (2011) Gene electrotransfer to tumor. In: Kee ST, Gehl J, Lee EW (eds) Clinical aspects of electroporation, 1st edn. Springer, New York, pp 159–164

    Chapter  Google Scholar 

  5. Daud AI, DeConti RC, Andrews S et al (2008) Phase I trial of interleukin-12 plasmid electroporation in patients with metastatic melanoma. J Clin Oncol 26(36):5896–5903

    CAS  PubMed Central  PubMed  Google Scholar 

  6. Cheresh DA, Stupack DG (2008) Regulation of angiogenesis: apoptotic cues from the ECM. Oncogene 27(48):6285–6298

    Article  CAS  PubMed  Google Scholar 

  7. Avraamides CJ, Garmy-Susini B, Varner JA (2008) Integrins in angiogenesis and lymphangiogenesis. Nat Rev Cancer 8(8):604–617

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  8. Brooks PC, Clark RA, Cheresh DA (1994) Requirement of vascular integrin alpha v beta 3 for angiogenesis. Science 264(5158):569–571

    Article  CAS  PubMed  Google Scholar 

  9. Johnson JP (1999) Cell adhesion molecules in the development and progression of malignant melanoma. Cancer Metastasis Rev 18(3):345–357

    Article  CAS  PubMed  Google Scholar 

  10. Kuphal S, Bauer R, Bosserhoff AK (2005) Integrin signaling in malignant melanoma. Cancer Metastasis Rev 24(2):195–222

    Article  CAS  PubMed  Google Scholar 

  11. Trochon-Joseph V, Martel-Renoir D, Mir LM et al (2004) Evidence of antiangiogenic and antimetastatic activities of the recombinant disintegrin domain of metargidin. Cancer Res 64(6):2062–2069

    Article  CAS  PubMed  Google Scholar 

  12. Spanggaard I, Snoj M, Cavalcanti A et al (2013) Gene electrotransfer of plasmid antiangiogenic metargidin peptide (AMEP) in disseminated melanoma: safety and efficacy results of a phase I first-in-man study. Hum Gene Ther Clin Dev 24(3):99–107

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Julie Gehl M.D., Dr.Med.Sci. .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer Science+Business Media New York

About this protocol

Cite this protocol

Spanggaard, I., Gehl, J. (2015). Antiangiogenic Metargidin Peptide (AMEP) Gene Therapy in Disseminated Melanoma. In: Walther, W., Stein, U. (eds) Gene Therapy of Solid Cancers. Methods in Molecular Biology, vol 1317. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2727-2_20

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-2727-2_20

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2726-5

  • Online ISBN: 978-1-4939-2727-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics