Abstract
The study of protein uptake into mitochondria is an important tool for investigating the subcellular distribution of proteins and the molecular mechanisms that determine location. Here we describe five techniques that allow the quantitative or qualitative monitoring of protein uptake into mitochondria using both in vitro and in vivo approaches.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Tanz SK, Castleden I, Hooper CM, Vacher M, Small I, Millar HA (2013) SUBA3: a database for integrating experimentation and prediction to define the SUBcellular location of proteins in Arabidopsis. Nucleic Acids Res 41(Database issue):D1185–D1191
Narsai R, Devenish J, Castleden I, Narsai K, Xu L, Shou H, Whelan J (2013) Rice DB: an Oryza Information Portal linking annotation, subcellular location, function, expression, regulation, and evolutionary information for rice and Arabidopsis. Plant J 76:1057–1073
Sambrook JFEFMT (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Murcha MW, Elhafez D, Millar AH, Whelan J (2005) The C-terminal region of TIM17 links the outer and inner mitochondrial membranes in Arabidopsis and is essential for protein import. J Biol Chem 280:16476–16483
Carrie C, Small I (2013) A reevaluation of dual-targeting of proteins to mitochondria and chloroplasts. Biochim Biophys Acta 1833:253–259
Rudhe C, Chew O, Whelan J, Glaser E (2002) A novel in vitro system for simultaneous import of precursor proteins into mitochondria and chloroplasts. Plant J 30:213–220
Aronsson H, Jarvis RP (2011) Rapid isolation of Arabidopsis chloroplasts and their use for in vitro protein import assays. Methods Mol Biol 774:281–305
Taylor NL, Stroher E, Millar AH (2014) Arabidopsis organelle isolation and characterization. Methods Mol Biol 1062:551–572
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Duncan, O., Carrie, C., Wang, Y., Murcha, M.W. (2015). In Vitro and In Vivo Protein Uptake Studies in Plant Mitochondria. In: Whelan, J., Murcha, M. (eds) Plant Mitochondria. Methods in Molecular Biology, vol 1305. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2639-8_5
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2639-8_5
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2638-1
Online ISBN: 978-1-4939-2639-8
eBook Packages: Springer Protocols