Abstract
A gene transfer technique by electroporation for the living embryo has become a standard method to analyze functions of genes in vivo. Combined with the well-established classical experimental embryological techniques for avian embryos in ovo, this methodology has made tremendous contributions to our understanding of the molecular and genetic mechanisms underlying development of the central nervous system. Based on the simple and straightforward principle of the method, one can now introduce DNA, siRNA, shRNA, and antisense oligonucleotides to cells/tissues of the living embryo in a temporally and spatially controlled manner, which enables a wide range of detailed, rapid and relatively inexpensive analyses of gene functions. We describe several examples of its application to the chick embryonic CNS, which would be useful for the related researches in the field.
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Acknowledgements
The authors thank Drs. H. Nakamura and K. Yasuda for introducing the original in ovo electroporation method for the first time, and Drs. Y. Takahashi and S. Nakagawa for the Tol2 and Tet-ON, -OFF systems. We also thank Y. Hayakawa for letting us use NEPA21. This work was supported by grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
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Hatakeyama, J., Shimamura, K. (2015). Electroporation for the Chick Embryonic CNS. In: Saito, T. (eds) Electroporation Methods in Neuroscience. Neuromethods, vol 102. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2459-2_9
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DOI: https://doi.org/10.1007/978-1-4939-2459-2_9
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