Abstract
Retroviral transduction is an invaluable technique in molecular biology used to express proteins encoded by nonviral genes in mammalian cells. A key feature of this technique is the ability to create cell lines that stably express the protein of interest and can be cultured long term. Here we describe a retroviral transduction procedure for mouse embryonic fibroblasts (MEFs) that uses Platinum-E cells to rapidly package high-titer, helper-free retrovirus. This technique is useful to study the role of key signaling kinases in the NF-κB signal transduction pathway.
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Gray, C.M., May, M.J. (2015). Stable Reconstitution of IKK-Deficient Mouse Embryonic Fibroblasts. In: May, M. (eds) NF-kappa B. Methods in Molecular Biology, vol 1280. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2422-6_10
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DOI: https://doi.org/10.1007/978-1-4939-2422-6_10
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2421-9
Online ISBN: 978-1-4939-2422-6
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