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High-Resolution Atomic Force Microscopy Imaging of Rhodopsin in Rod Outer Segment Disk Membranes

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Rhodopsin

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1271))

Abstract

Atomic force microscopy (AFM) is a powerful imaging technique that allows recording topographical information of membrane proteins under near-physiological conditions. Remarkable results have been obtained on membrane proteins that were reconstituted into lipid bilayers. High-resolution AFM imaging of native disk membranes from vertebrate rod outer segments has unveiled the higher-order oligomeric state of the G protein-coupled receptor rhodopsin, which is highly expressed in disk membranes. Based on AFM imaging, it has been demonstrated that rhodopsin assembles in rows of dimers and paracrystals and that the rhodopsin dimer is the fundamental building block of higher-order structures.

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Acknowledgements

Financial support from the University of Bern, the Bern University Research Foundation, the Swiss National Science Foundation, and the National Centres of Competence in Research (NCCR) TransCure and Molecular Systems Engineering is gratefully acknowledged.

Author information

Authors and Affiliations

  1. Institute of Biochemistry and Molecular Medicine, University of Bern, Bühlstrasse 28, Bern, CH-3012, Switzerland

    Patrick D. Bosshart & Dimitrios Fotiadis Ph.D.

  2. Swiss National Centre of Competence in Research (NCCR) TransCure, University of Bern, Bern, CH-3012, Switzerland

    Patrick D. Bosshart & Dimitrios Fotiadis Ph.D.

  3. Department of Pharmacology, School of Medicine, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH, 44106-4965, USA

    Andreas Engel

  4. Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, 2628 CJ, The Netherlands

    Andreas Engel

Authors
  1. Patrick D. Bosshart
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  2. Andreas Engel
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  3. Dimitrios Fotiadis Ph.D.
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Corresponding author

Correspondence to Dimitrios Fotiadis Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA

    Beata Jastrzebska

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Bosshart, P.D., Engel, A., Fotiadis, D. (2015). High-Resolution Atomic Force Microscopy Imaging of Rhodopsin in Rod Outer Segment Disk Membranes. In: Jastrzebska, B. (eds) Rhodopsin. Methods in Molecular Biology, vol 1271. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2330-4_13

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  • DOI: https://doi.org/10.1007/978-1-4939-2330-4_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2329-8

  • Online ISBN: 978-1-4939-2330-4

  • eBook Packages: Springer Protocols

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