Abstract
Subcellular fractionation is a valuable tool to follow protein traffic between cellular compartments. Here we detail a procedure for fractionating erythrocytes infected with the human malaria parasite P. falciparum using the bacterial pore-forming protein Streptolysin O (SLO). Additionally we describe an experimental protocol to determine protein topology by carrying out a protease protection assay on SLO-lysed infected erythrocytes.
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Acknowledgement
J.M.P. is supported by DFG Grant PR1099/3-1 as part of the SPP1580. S.K. is supported by a postdoctoral DFG scholarship.
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Külzer, S., Bittl, V., Przyborski, J.M. (2015). Fractionation of Plasmodium-Infected Human Red Blood Cells to Study Protein Trafficking. In: Tang, B. (eds) Membrane Trafficking. Methods in Molecular Biology, vol 1270. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2309-0_5
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DOI: https://doi.org/10.1007/978-1-4939-2309-0_5
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