Abstract
mRNA binding proteins regulate gene expression by controlling the processing, localization, decay, and translation of messenger RNAs (mRNAs). To fully understand this process, it is necessary to identify the complete set of mRNA binding proteins. This work describes a method for the systematic identification of yeast mRNA binding proteins. This method applies in vivo UV cross-linking, affinity pull-down of polyA(+) mRNAs, and analysis by mass spectrometry to identify proteins that directly bind to mRNAs.
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Mitchell, S.F., Parker, R. (2015). In Vivo Cross-Linking Followed by PolyA Enrichment to Identify Yeast mRNA Binding Proteins. In: Boudvillain, M. (eds) RNA Remodeling Proteins. Methods in Molecular Biology, vol 1259. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2214-7_3
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DOI: https://doi.org/10.1007/978-1-4939-2214-7_3
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2213-0
Online ISBN: 978-1-4939-2214-7
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