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Silencing of E6/E7 Expression in Cervical Cancer Stem-Like Cells

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Cervical Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1249))

Abstract

Accumulating evidence supports the concept that cancer stem cells (CSCs) are responsible for the tumor recurrence and metastasis, the two major causes of cancer-related death. Therefore, CSC-targeted cancer therapy is important for the future development of more effective and advanced cancer therapy. One of the approaches is to specifically silence oncogene expression in CSCs and inhibit their growth. The significance of this approach is its specificity and ability to avoid multi-drug resistance of CSCs. In this chapter, we will describe a method of silencing HPV oncogenes E6/E7 in human cervical CSCs using HeLa cells as a model system.

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Author information

Authors and Affiliations

  1. Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Corner College and Cooper Roads (Building 75), Brisbane, St. Lucia, QLD, 4072, Australia

    Wenyi Gu & Chengzhong Yu

  2. Cancer Research Centre, Griffith Health Institute, School of Medical Science, Griffith University, Southport, QLD, Australia

    Nigel McMillan

Authors
  1. Wenyi Gu
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  2. Nigel McMillan
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  3. Chengzhong Yu
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Corresponding author

Correspondence to Chengzhong Yu .

Editor information

Editors and Affiliations

  1. Touro University California, Vallejo, California, USA

    Daniel Keppler

  2. Touro University California, Vallejo, California, USA

    Athena W. Lin

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Gu, W., McMillan, N., Yu, C. (2015). Silencing of E6/E7 Expression in Cervical Cancer Stem-Like Cells. In: Keppler, D., Lin, A. (eds) Cervical Cancer. Methods in Molecular Biology, vol 1249. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2013-6_12

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  • DOI: https://doi.org/10.1007/978-1-4939-2013-6_12

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2012-9

  • Online ISBN: 978-1-4939-2013-6

  • eBook Packages: Springer Protocols

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